PMID: 517268Jan 1, 1979Paper

Autoradiographic studies on the cellular localization of GABA and beta-alanine uptake by neurones and glia in tissue culture

Advances in Experimental Medicine and Biology
L Hösli, E Hösli

Abstract

Nervous tissue cultures have proved to be an excellent tool for investigating the cellular localization of the uptake of transmitter substances using autoradiography. The uptake of [3H]GABA and [3H]beta-alanine was studied in cultures of cerebellum, brain stem, spinal cord and dorsal root ganglia (DRG) of the rat. In cultures of brain stem and spinal cord, [3H]GABA was taken up by a great number of neurones and by almost all glial cells, suggesting that glial elements might also be involved in the inactivation of this amino acid. In cerebellar cultures, [3H]GABA was accumulated by many interneurones, by Purkinje cells and by a great number of glial cells. The finding that cultured Purkinje cells accumulated [3H]GABA contrasts with observations made in the cerebellum in vivo or in slices where no labelling of Purkinje cells could be demonstrated. Since GABA is taken up to a great extent by Bergman glia which tightly surround Purkinje cells in vivo, it has been suggested that the barrier formed by these glial cells prevents the uptake of the amino acid into Purkinje cells. In cultured cerebellum this glial barrier might be disrupted or absent and therefore Purkinje cells are able to accumulate [3H]GABA. A similar glial barrier pr...Continue Reading

Citations

Mar 24, 2004·The Journal of Biological Chemistry·Tokuyuki ShinoharaShuji Hinuma
Nov 1, 1981·The Journal of Comparative Neurology·R R MizeP Sterling
Aug 11, 1981·Molecular and Cellular Biochemistry·R L Macdonald, A B Young
Mar 10, 2001·Journal of Neuroscience Research·A Gadea, A M López-Colomé
Jan 1, 1982·Psychoneuroendocrinology·A N EliasM K Grossman

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