Autoradiography and inmmunolabeling suggests that lizard blastema contains arginase-positive M2-like macrophages that may support tail regeneration.

Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft
Lorenzo Alibardi

Abstract

The regenerating blastema of the tail in the lizard Podarcis muralis contains numerous macrophages among the prevalent mesenchymal cells. Some macrophages are phagocytic but others are devoid of phagosomes suggesting that they have other roles aside phagocytosis. The presence of healing macrophages (M2-like) has been tested using autoradiographic, immunohistochemical and ultrastructural studies. Autoradiography shows an uptake of tritiated arginine in sparse cells of the blastema and in the regenerating epidermis. Bioinformatics analysis suggests that epitopes for arginase-1 and -2, recognized by the employed antibody, are present in lizards. Immunofluorescence shows sparse arginase immunopositive macrophages in the blastema and few macrophages also in the apical wound epidermis. The ultrastructural study shows that macrophages contain dense secretory granules, most likely inactive lysosomes, and small cytoplasmic pale vesicles. Some of the small vesicles are arginase-positive while immunolabeling is very diffuse in the macrophage cytoplasm. The presence of cells incorporating arginine and of arginase 1-positive cells suggests that M2-like macrophages are present among mesenchymal and epidermal cells of the regenerative tail bl...Continue Reading

References

Nov 1, 1992·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·C ScalaG C Manara
Aug 12, 1998·American Journal of Respiratory Cell and Molecular Biology·R M SchapiraL D Nelin
May 4, 2007·The Journal of Clinical Investigation·Antonio Sica, Vincenzo Bronte
Nov 26, 2008·Nature Reviews. Immunology·David M Mosser, Justin P Edwards
Sep 22, 2009·British Journal of Pharmacology·Markus Munder
Feb 16, 2010·The Journal of Experimental Biology·L M ZimmermanR M Bowden
Jul 12, 2011·Expert Reviews in Molecular Medicine·Timothy J Koh, Luisa Ann DiPietro
Aug 19, 2011·BMC Developmental Biology·Katherine E McLean, Matthew K Vickaryous
Mar 2, 2012·The Journal of Clinical Investigation·Antonio Sica, Alberto Mantovani
Mar 23, 2012·Nature Reviews. Immunology·Dmitry I GabrilovichVincenzo Bronte
Aug 31, 2012·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Rebecca E FisherJeanne Wilson-Rawls
Jan 4, 2013·PloS One·Kristian W SanggaardJan J Enghild
May 22, 2013·Proceedings of the National Academy of Sciences of the United States of America·James W GodwinNadia A Rosenthal
Nov 19, 2013·Physiological and Biochemical Zoology : PBZ·Emily A B GilbertMatthew K Vickaryous
Feb 26, 2014·Differentiation; Research in Biological Diversity·James W Godwin, Nadia Rosenthal
Feb 20, 2016·Developmental and Comparative Immunology·Anthony L MescherMichael W King
Jul 28, 2016·Connective Tissue Research·Thomas P Lozito, Rocky S Tuan
Aug 16, 2016·Seminars in Cell & Developmental Biology·James W GodwinNadia A Rosenthal
Nov 22, 2016·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Nicola VituloLorenzo Alibardi
May 17, 2017·ELife·Jennifer SimkinAshley W Seifert
Jun 15, 2017·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Lorenzo Alibardi
Nov 14, 2018·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Lorenzo Alibardi

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