PMID: 5909582Mar 25, 1966

Autoradiography with tritiated methotrexate and the cellular distribution of folate reductase

Science
Z DarzynkiewiczW C Werkheiser

Abstract

Bound methotrexate has been revealed by an autoradiographic procedure, presumed to introduce a method for cytochemical study of folate reductase. Preferential localization is seen in kidney proximal tubules, intestinal epithelium, and nuclei of parenchymal liver cells in mice. The extremely firm binding and prolonged retention of this drug should render it suitable as an inert label for the autoradiographic study of cell migrations and lifetimes.

Citations

Sep 1, 1972·The Histochemical Journal·K Ostrowski
Sep 1, 1967·British Journal of Haematology·V M Whitehead, B A COOPER
Mar 1, 1968·Biochemical Pharmacology·B G GunlackD C Williams
Jun 9, 2020·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Z Darzynkiewicz

Related Concepts

Autoradiography
Intestines, Small
Kidney
Liver
Methotrexate, (DL)-Isomer
Tetrahydrofolate Dehydrogenase
Tritium

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