Autoregulation by cooperative binding of the PemI and PemK proteins to the promoter region of the pem operon

Molecular & General Genetics : MGG
S Tsuchimoto, E Ohtsubo

Abstract

The low copy number plasmid R100 carries the pem region, consisting of two genes, pemI and pemK, which are required for stable maintenance of the plasmid. Here, to understand the regulation of the expression of the pem region, we constructed plasmids carrying either the pemI or the pemK gene, whose initiation codons were fused in frame with the lacZ gene, and examined their expression by assaying beta-galactosidase (LacZ) activity. The synthesis of both PemI and PemK proteins was found to be repressed coordinately in the presence of a plasmid carrying the entire pem region. This indicates that pemK and pemI cistrons form an operon, and that the expression of the operon is negatively regulated by its own products. We then conducted a gel retardation assay in vitro and found that the two pem products, each of which was obtained as a tripartite protein (PemI-collagen-LacZ and PemK-collagen-LacZ), bound cooperatively to a specific fragment containing the proximal region of the pem operon. The binding region, determined by DNase I footprinting analysis, included the promoter for the pem operon. This indicates that both PemI and PemK proteins bind to the promoter region to autoregulate their synthesis.

References

Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·A D JohnsonM Ptashne
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Oct 1, 1991·Journal of Bacteriology·T AboE Ohtsubo
Sep 1, 1989·Molecular & General Genetics : MGG·R de FeyterD Lane
May 1, 1989·Journal of Bacteriology·J E Tam, B C Kline
Feb 1, 1989·Molecular & General Genetics : MGG·S Tsuchimoto, E Ohtsubo
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·H IchikawaE Ohtsubo
Apr 1, 1988·Journal of Bacteriology·S TsuchimotoE Ohtsubo
Jun 5, 1988·Journal of Molecular Biology·J H NewM Fennewald
Jan 1, 1986·Advances in Biophysics·H OhtsuboE Ohtsubo
Oct 1, 1986·Molecular & General Genetics : MGG·K A ArmstrongE Ohtsubo
Jan 1, 1987·Methods in Enzymology·J Vieira, J Messing
Aug 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J Germino, D Bastia
Jan 1, 1980·Methods in Enzymology·A M Maxam, W Gilbert
Apr 1, 1980·Journal of Molecular Biology·M J Casadaban, S N Cohen

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Citations

Aug 23, 2003·Plasmid·Sandra Santos-SierraRamon Díaz-Orejas
Jul 15, 2006·Molecular Plant-microbe Interactions : MPMI·Monica BodogaiIlona Dusha
Jul 19, 2011·Journal of Bacteriology·Wai Ting ChanChew Chieng Yeo
Mar 4, 2005·Journal of Bacteriology·Xueyan Zhao, Roy David Magnuson
Jun 29, 2006·Molecular Microbiology·Ciarán Condon
Nov 13, 2003·BMC Microbiology·Philip JordanNigel J Saunders
Nov 5, 1999·Annual Review of Microbiology·H Engelberg-Kulka, G Glaser
Aug 7, 2007·Genome Biology·Emeric W Sevin, Frédérique Barloy-Hubler
Mar 28, 2009·PLoS Genetics·Laurence Van Melderen, Manuel Saavedra De Bast
Jan 18, 2006·Journal of Molecular Biology·Monique B KamphuisRolf Boelens
Jan 15, 2020·Journal of Bacteriology·Nathan FraikinLaurence Van Melderen
Jun 6, 2008·Molecular Microbiology·Martin OvergaardKenn Gerdes
Jan 25, 2011·Microbial Biotechnology·Jens KrollAlexander Steinbüchel
Feb 15, 2014·RNA Biology·Jason M Schifano, Nancy A Woychik
Jun 13, 2020·Frontiers in Microbiology·Jessica R AmesChristina R Bourne
Feb 20, 2020·Scientific Reports·Charlie Y HuangKathleen R Ryan
Mar 17, 2004·The Journal of Biological Chemistry·Junjie ZhangMasayori Inouye
Aug 2, 1996·The Journal of Biological Chemistry·R MagnusonM B Yarmolinsky
Jun 18, 2003·The Journal of Biological Chemistry·Junjie ZhangMasayori Inouye
Nov 10, 2000·The Journal of Biological Chemistry·I MarianovskyG Glaser
Nov 26, 1998·Journal of Bacteriology·R Magnuson, M B Yarmolinsky

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