Autoregulatory loop and retinoic acid repression regulate pou2/pou5f1 gene expression in the zebrafish embryonic brain

Developmental Dynamics : an Official Publication of the American Association of Anatomists
Mst Shahnaj ParvinKyo Yamasu

Abstract

Zebrafish pou5f1, also known as pou2, encodes a POU-family transcription factor that is transiently expressed in the prospective midbrain and anterior hindbrain during gastrulation, governing brain development. In the present study, we found that the main regulatory elements reside in the proximal upstream DNA sequence from -2.2 to -0.12 kb (the -2.2/-0.1 region). The electrophoretic gel mobility shift assay (EMSA) revealed four functional octamer sequences that can associate with zebrafish Pou2/Pou5f1. The expression of mutated reporter constructs, as well as EMSA, suggested that these four octamer sequences operate in a cooperative manner to drive expression in the mid/hindbrain. We also identified a retinoic acid (RA)-responsive element in this proximal region, which was required to repress transcription in the posterior part of the embryo. These data provide a scheme wherein pou2/pou5f1 expression in zebrafish embryos is regulated by both an autoregulatory loop and repression by RA emanating from the posterior mesoderm.

References

Nov 1, 1991·Mechanisms of Development·Y I YeomK Artzt
May 1, 1995·Mechanisms of Development·G Hauptmann, T Gerster
Jul 1, 1995·Developmental Dynamics : an Official Publication of the American Association of Anatomists·C B KimmelT F Schilling
Nov 20, 1998·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M PesceH R Schöler
Dec 3, 1998·Molecular Human Reproduction·C E Ovitt, H R Schöler
Feb 23, 1999·Advances in Experimental Medicine and Biology·M D SchonemannM G Rosenfeld
Jul 14, 2000·Physiological Reviews·S A RossL M De Luca
Feb 17, 2001·Current Opinion in Neurobiology·M Rhinn, M Brand
Feb 24, 2001·Journal of Molecular Biology·K Phillips, B Luisi
Jul 21, 2001·Stem Cells·M Pesce, H R Schöler
Mar 14, 2002·Proceedings of the National Academy of Sciences of the United States of America·Ying GuoRobert Hromas
May 2, 2002·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Cecilia B Moens, Victoria E Prince
Sep 10, 2003·Mechanisms of Development·Muriel RhinnMichael Brand
Oct 28, 2003·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Hiroshi KikutaKyo Yamasu
Feb 6, 2004·The Journal of Biological Chemistry·Naoko HattoriKunio Shiota
Nov 24, 2004·The Journal of Biological Chemistry·Sayaka Okumura-NakanishiFuyuki Ishikawa
Dec 8, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Verónica Ramos-MejíaHilda Lomelí
Aug 23, 2005·Brain Research. Brain Research Reviews·Matías Hidalgo-SánchezRosa-Magda Alvarado-Mallart
Jan 13, 2006·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Yuich OkudaYusuke Kamachi
Feb 2, 2006·Gene Expression Patterns : GEP·Alexandra TallafussJohn H Postlethwait
Feb 4, 2006·Gene Expression Patterns : GEP·Laura A HaleJohn H Postlethwait
Sep 12, 2006·Development, Growth & Differentiation·Fumitaka InoueKyo Yamasu
Nov 10, 2006·Nature·Jianlong WangStuart H Orkin
Dec 5, 2006·Methods in Enzymology·Lianchun Fan, Paul Collodi
Jan 12, 2007·Development·Hitoshi Niwa

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Citations

Dec 31, 2010·Biology Direct·Georg Fuellen, Stephan Struckmann
Apr 14, 2016·In Vitro Cellular & Developmental Biology. Animal·Shinpei YokotaTohru Suzuki
Dec 18, 2014·In Vitro Cellular & Developmental Biology. Animal·Hiroyuki KatoTohru Suzuki
Jan 7, 2015·Nature Reviews. Molecular Cell Biology·Thomas J Cunningham, Gregg Duester

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