Autosomal SNP typing of forensic samples with the GenPlex™ HID System: results of a collaborative study

Forensic Science International. Genetics
Carmen TomasNiels Morling

Abstract

The GenPlex™ HID System (Applied Biosystems - AB) offers typing of 48 of the 52 SNPforID SNPs and amelogenin. Previous studies have shown a high reproducibility of the GenPlex™ HID System using 250-500pg DNA of good quality. An international exercise was performed by 14 laboratories (9 in Europe and 5 in the US) in order to test the robustness and reliability of the GenPlex™ HID System on forensic samples. Three samples with partly degraded DNA and 10 samples with low amounts of DNA were analyzed in duplicates using various amounts of DNA. In order to compare the performance of the GenPlex™ HID System with the most commonly used STR kits, 500pg of partly degraded DNA from three samples was typed by the laboratories using one or more STR kits. The median SNP typing success rate was 92.3% with 500pg of partly degraded DNA. Three of the fourteen laboratories counted for more than two thirds of the locus dropouts. The median percentage of discrepant results was 0.2% with 500pg degraded DNA. An increasing percentage of locus dropouts and discrepant results were observed when lower amounts of DNA were used. Different success rates were observed for the various SNPs. The rs763869 SNP was the least successful. With the exception of the...Continue Reading

References

Apr 6, 2006·Electrophoresis·Juan J SanchezNiels Morling
Jun 1, 2007·Forensic Science International. Genetics·Esther Musgrave-BrownDenise Syndercombe Court
Dec 17, 2008·Forensic Science International. Genetics·J J SanchezN Morling
Dec 17, 2008·Forensic Science International. Genetics·Claus BørstingNiels Morling
Dec 17, 2008·Forensic Science International. Genetics·Carmen TomasUNKNOWN SNPforID Consortium
Dec 2, 2009·Forensic Science International. Genetics·Kaarel KrjutskovAndres Metspalu

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Citations

Jun 6, 2013·International Journal of Legal Medicine·Seung Bum SeoBruce Budowle
Dec 10, 2013·Forensic Science International. Genetics·Jing JiaCai-Xia Li
Aug 6, 2003·Journal of Pharmaceutical and Biomedical Analysis·C SlijkhuisD de Kaste
Aug 2, 2012·Transfusion Medicine and Hemotherapy : Offizielles Organ Der Deutschen Gesellschaft Fur̈ Transfusionsmedizin Und Immunham̈atologie·Claus BørstingNiels Morling
Mar 26, 2013·Forensic Science International. Genetics·Claus BørstingNiels Morling
Mar 27, 2012·Forensic Science International. Genetics·Kenneth K KiddAndrew J Pakstis
Mar 7, 2012·Journal of Forensic Sciences·Zhaoshu ZengZiming Dong
Nov 20, 2014·International Journal of Legal Medicine·David H WarshauerBruce Budowle
Jan 16, 2018·Trauma, Violence & Abuse·Joy OrpinShuby Puthussery
Jul 5, 2013·Journal of Forensic Sciences·James W SchummRichard Selden

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