Autotransplantation of the spleen in the rat: donor leukocytes of the splenic fragment survive implantation to migrate and proliferate in the host

Cell and Tissue Research
J Westermann, R Pabst

Abstract

Loss of the spleen may lead to fatal bacterial infections. As a preventive procedure splenic autotransplantation has been performed in humans and experimental animals. However, there is still controversy about the protective function of this procedure, partly because the process of regeneration after implantation of splenic tissue is not fully understood. In the present study the question was addressed of whether, in contrast to the current view, leukocytes survive the phase of necrosis after implantation of splenic fragments. Rats (LEW.7A; host) received splenic fragments of a congenic rat strain (LEW.7B; donor). These fragments first underwent almost complete necrosis, then regenerated, finally developing the typical splenic compartments. Twenty weeks after implantation, leukocytes which had survived the implantation procedure (7B positive; donor) and those which had migrated from the host into the splenic implant (7B negative; host) were differentiated using a specific monoclonal antibody (anti-7B) and immunohistology. In addition, the rats received 5-bromo-2-deoxyuridine (BrdU) 1 day before the splenic autotransplant and several lymphoid and non-lymphoid organs were removed. This thymidine analogue is incorporated in prolif...Continue Reading

Citations

Jan 25, 2005·Bulletin of Experimental Biology and Medicine·I N NifontovaI L Chertkov
Dec 6, 2000·The Journal of Small Animal Practice·D M AndersonR A White
Sep 27, 2000·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·M S ExtonM Schedlowski

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