Autotrophic and heterotrophic acquisition of carbon and nitrogen by a mixotrophic chrysophyte established through stable isotope analysis

The ISME Journal
Ramon TerradoDavid A Caron

Abstract

Collectively, phagotrophic algae (mixotrophs) form a functional continuum of nutritional modes between autotrophy and heterotrophy, but the specific physiological benefits of mixotrophic nutrition differ among taxa. Ochromonas spp. are ubiquitous chrysophytes that exhibit high nutritional flexibility, although most species generally fall towards the heterotrophic end of the mixotrophy spectrum. We assessed the sources of carbon and nitrogen in Ochromonas sp. strain BG-1 growing mixotrophically via short-term stable isotope probing. An axenic culture was grown in the presence of either heat-killed bacteria enriched with 15N and 13C, or unlabeled heat-killed bacteria and labeled inorganic substrates (13C-bicarbonate and 15N-ammonium). The alga exhibited high growth rates (up to 2 divisions per day) only until heat-killed bacteria were depleted. NanoSIMS and bulk IRMS isotope analyses revealed that Ochromonas obtained 84-99% of its carbon and 88-95% of its nitrogen from consumed bacteria. The chrysophyte assimilated inorganic 13C-carbon and 15N-nitrogen when bacterial abundances were very low, but autotrophic (photosynthetic) activity was insufficient to support net population growth of the alga. Our use of nanoSIMS represents its...Continue Reading

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Citations

Oct 8, 2019·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Susanne WilkenAlexandra Z Worden
Nov 7, 2019·Plant, Cell & Environment·Florent PenenDirk Schaumlöffel
Sep 29, 2017·Biointerphases·Jamie NuñezChristopher R Anderton
Nov 22, 2017·Environmental Microbiology·Alexis L PasulkaVictoria J Orphan
Feb 18, 2020·Journal of Applied Genetics·K K Nkongolo, R Narendrula-Kotha
Sep 24, 2020·Applied Microbiology and Biotechnology·Cheng CenRenjie Pan
Dec 3, 2020·Frontiers in Plant Science·K G Srikanta DaniFrancesco Loreto
Jan 29, 2020·Trends in Cell Biology·Johan DecelleNiculina Musat
Aug 1, 2021·Environmental Science and Pollution Research International·Rania IbrahimHubert Cabana

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Methods Mentioned

BETA
light microscopy

Software Mentioned

Look
NanoSIMS
MATLAB

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