Autotrophic hydrogen photoproduction by operation of carbon-concentrating mechanism in Chlamydomonas reinhardtii under sulfur deprivation condition

Journal of Biotechnology
Min Eui HongSang Jun Sim

Abstract

Under autotrophic conditions, starch plays an important role in establishing anoxic conditions during PSII-dependent hydrogen (H2) photoproduction in microalgae. This is because starch is the sole organic substrate during respiratory consumption of internal oxygen (O2) from PSII-dependent direct pathway. Herein, we propose a novel approach to further facilitate the internal starch synthesis of Chlamydomonas reinhardtii through the operation of carbon-concentrating mechanism (CCM) along with a two-stage process based on sulfur (S) deprivation, thereby resulting in enhanced anaerobic capacity during PSII-dependent H2 photoproduction. When CCM-induced cells were exposed to high levels of carbon dioxide (CO2) (5%, v/v) with S deprivation, internal levels of starch were significantly elevated by retaining a functional CCM with the boosted photosynthetic activity during 24h of O2 evolution phase (I) of S deprivation. Consequently, during H2 production phase of S deprivation at irradiance of 50μEm(-2)s(-1), the concentrations of starch and H2 in CCM-induced cells were remarkably enhanced by 65.0% and 218.9% compared to that of CCM-uninduced cells, respectively. The treatment of low-CO2-driven CCM induction prior to S deprivation is a ...Continue Reading

References

Nov 14, 2001·Plant Physiology·A Melis, T Happe
Feb 16, 2002·European Journal of Biochemistry·Thomas Happe, Annette Kaminski
Apr 15, 2004·The Journal of Biological Chemistry·Matthew C PosewitzMaria L Ghirardi
Oct 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J R Coleman, A R Grossman
Aug 10, 2006·Applied Microbiology and Biotechnology·Jens RupprechtOlaf Kruse
Jun 15, 2007·Eukaryotic Cell·James V Moroney, Ruby A Ynalvez
Nov 6, 2008·Biotechnology and Bioengineering·Irina V TolstyginaAnatoly A Tsygankov
Mar 2, 2010·Trends in Biotechnology·Hyung-Sool LeeBruce E Rittmann
Jul 5, 2011·Journal of Biotechnology·Alberto ScomaGiuseppe Torzillo
Jul 29, 2011·Trends in Biotechnology·Maria G EsquívelF Xavier Malcata
Apr 17, 2013·Proceedings of the National Academy of Sciences of the United States of America·Alena VolgushevaFikret Mamedov
Mar 15, 2015·The Plant Journal : for Cell and Molecular Biology·Yingjun WangMartin H Spalding

❮ Previous
Next ❯

Citations

Jun 4, 2020·Cells·Neda FakhimiAlexandra Dubini
Nov 25, 2021·Environmental Science and Pollution Research International·Karima TaziAbdellatif Janati Idrissi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cavernous Hemangioma

Cavernous hemangioma is a blood vessel defect or benign tumor that leads to leakage of blood to the surrounding tissues. This can occur in several organs including the brain, which can lead to seizures. Discover the latest research on cavernous hemangiomas here.