PMID: 2317515Mar 29, 1990

Autoxidation of myoglobin from bigeye tuna fish (Thunnus obesus)

Biochimica Et Biophysica Acta
Y KitaharaK Shikama

Abstract

Native oxymyoglobin (MbO2) was isolated directly from the skeletal muscle of bigeye tuna (Thunnus obesus) with complete separation from metmyoglobin (metMb) on a CM-cellulose column. It was examined for its stability properties over a wide range of pH values (pH 5-12) in 0.1 M buffer at 25 degrees C. When compared with sperm whale MbO2 as a reference, the tuna MbO2 was found to be much more susceptible to autoxidation. Kinetic analysis has revealed that the rate constant for a nucleophilic displacement of O2- from MbO2 by an entering water molecule is 10-times higher than the corresponding value for sperm whale MbO2. The magnitude of the circular dichroism of bigeye tuna myoglobin at 222 nm was comparable to that of sperm whale myoglobin, but its hydropathy profile revealed the region corresponding to the distal side of the heme iron to be apparently less hydrophobic. The kinetic simulation also demonstrated that accessibility of the solvent water molecule to the heme pocket is clearly a key factor in the stability properties of the bound dioxygen.

References

Jan 1, 1987·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·A MatsuokaK Shikama
Sep 14, 1971·Journal of Molecular Biology·E E LattmanW E Love
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Jul 18, 1980·Journal of Chromatography·T SuzukiK Shikama

Citations

Jul 12, 2005·Progress in Biophysics and Molecular Biology·K Shikama
Oct 5, 1990·European Journal of Biochemistry·Y TsubamotoK Shikama
Jul 18, 2014·Fukushima Journal of Medical Science·Gen KobayashiAriki Matsuoka
Nov 30, 2012·Annual Review of Food Science and Technology·Surendranath P Suman, Poulson Joseph
Apr 30, 2016·Fish Physiology and Biochemistry·Mala Nurilmala, Yoshihiro Ochiai
May 1, 1994·Biological Reviews of the Cambridge Philosophical Society·K Shikama, A Matsuoka
Oct 26, 2005·Bioscience, Biotechnology, and Biochemistry·Nobuhiko Ueki, Yoshihiro Ochiai
May 1, 2007·The Journal of Pharmacology and Experimental Therapeutics·Pierantonio MennaGiorgio Minotti
Apr 19, 2018·Journal of Food Science and Technology·Mala NurilmalaYoshihiro Ochiai

Related Concepts

Circular Dichroism, Vibrational
Heme
Hydrogen-Ion Concentration
Muscle
Myoglobin
Oxidation-Reduction
Structure-Activity Relationship
Tuna (fish)

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