PMID: 2317515Mar 29, 1990

Autoxidation of myoglobin from bigeye tuna fish (Thunnus obesus)

Biochimica Et Biophysica Acta
Y KitaharaK Shikama


Native oxymyoglobin (MbO2) was isolated directly from the skeletal muscle of bigeye tuna (Thunnus obesus) with complete separation from metmyoglobin (metMb) on a CM-cellulose column. It was examined for its stability properties over a wide range of pH values (pH 5-12) in 0.1 M buffer at 25 degrees C. When compared with sperm whale MbO2 as a reference, the tuna MbO2 was found to be much more susceptible to autoxidation. Kinetic analysis has revealed that the rate constant for a nucleophilic displacement of O2- from MbO2 by an entering water molecule is 10-times higher than the corresponding value for sperm whale MbO2. The magnitude of the circular dichroism of bigeye tuna myoglobin at 222 nm was comparable to that of sperm whale myoglobin, but its hydropathy profile revealed the region corresponding to the distal side of the heme iron to be apparently less hydrophobic. The kinetic simulation also demonstrated that accessibility of the solvent water molecule to the heme pocket is clearly a key factor in the stability properties of the bound dioxygen.


Jan 1, 1987·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·A MatsuokaK Shikama
Sep 14, 1971·Journal of Molecular Biology·E E LattmanW E Love
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Jul 18, 1980·Journal of Chromatography·T SuzukiK Shikama


Jul 12, 2005·Progress in Biophysics and Molecular Biology·K Shikama
Oct 5, 1990·European Journal of Biochemistry·Y TsubamotoK Shikama
Jul 18, 2014·Fukushima Journal of Medical Science·Gen KobayashiAriki Matsuoka
Nov 30, 2012·Annual Review of Food Science and Technology·Surendranath P Suman, Poulson Joseph
Apr 30, 2016·Fish Physiology and Biochemistry·Mala Nurilmala, Yoshihiro Ochiai
May 1, 1994·Biological Reviews of the Cambridge Philosophical Society·K Shikama, A Matsuoka
Oct 26, 2005·Bioscience, Biotechnology, and Biochemistry·Nobuhiko Ueki, Yoshihiro Ochiai
May 1, 2007·The Journal of Pharmacology and Experimental Therapeutics·Pierantonio MennaGiorgio Minotti
Apr 19, 2018·Journal of Food Science and Technology·Mala NurilmalaYoshihiro Ochiai

Related Concepts

Circular Dichroism, Vibrational
Hydrogen-Ion Concentration
Structure-Activity Relationship
Tuna (fish)

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Synthetic Genetic Array Analysis

Synthetic genetic arrays allow the systematic examination of genetic interactions. Here is the latest research focusing on synthetic genetic arrays and their analyses.

Congenital Hyperinsulinism

Congenital hyperinsulinism is caused by genetic mutations resulting in excess insulin secretion from beta cells of the pancreas. Here is the latest research.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Epigenetic Memory

Epigenetic memory refers to the heritable genetic changes that are not explained by the DNA sequence. Find the latest research on epigenetic memory here.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Femoral Neoplasms

Femoral Neoplasms are bone tumors that arise in the femur. Discover the latest research on femoral neoplasms here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.