Auxin Induces Widespread Proteome Remodeling in Arabidopsis Seedlings

Proteomics
Natalie M ClarkDior R Kelley

Abstract

It is known that auxin induces rapid gene expression changes throughout plant development, but how these transcriptional responses relate to changes in protein abundance is not well characterized. This report identifies early auxin responsive proteins in whole Arabidopsis seedlings using an isobaric tags for relative and absolute quantification-based quantitative proteomics approach. Approximately 25% of the detected proteins (1045 out of 4257 proteins) are auxin responsive, which is in line with the central role of auxin in the regulation of plant growth and development. Several well-known auxin pathway proteins are identified as differentially expressed, validating this quantitative proteomics approach. Additionally, functional categorization of these auxin responsive proteins indicates that rapid and complex metabolic changes occur in seedlings in response to auxin, including lipid metabolism. Altogether, these data describe novel auxin-regulated proteins and are an excellent resource for identifying new downstream signaling components related to auxin-mediated plant growth and development.

References

Mar 11, 2003·Proceedings of the National Academy of Sciences of the United States of America·Michael P WashburnJohn R Yates
Oct 17, 2003·Nature·Sina GhaemmaghamiJonathan S Weissman
Oct 16, 2004·The Plant Cell·Ranjan SwarupMalcolm J Bennett
Apr 9, 2005·Trends in Plant Science·Ivan A PaponovKlaus Palme
Jul 17, 2007·The Plant Cell·Anna N StepanovaJose M Alonso
May 20, 2011·Nature·Björn SchwanhäusserMatthias Selbach
Jun 23, 2011·PLoS Genetics·Anatole GhazalpourAldons J Lusis
Nov 9, 2011·Plant Physiology·Barry CausierBrendan Davies
Mar 1, 2012·The Plant Cell·Rocío González-LamotheKamal Bouarab
Jun 13, 2012·Developmental Biology·Cédric Finet, Yvon Jaillais
Jul 21, 2012·Acta Biochimica Et Biophysica Sinica·Meiqing Xing, Hongwei Xue
Sep 12, 2013·Molecular Systems Biology·Bastiaan O R BargmannKenneth D Birnbaum
Sep 17, 2013·Journal of Proteome Research·Benedetta MatteiM Eugenia Schininà
Nov 20, 2013·Proceedings of the National Academy of Sciences of the United States of America·Justin W WalleySteven P Briggs
Feb 20, 2014·The Plant Journal : for Cell and Molecular Biology·Lalit PonnalaKlaas J van Wijk
Jul 26, 2014·The Plant Journal : for Cell and Molecular Biology·Joanne E LeeJohn F Golz
Apr 30, 2015·Proceedings of the National Academy of Sciences of the United States of America·Justin WalleyKatayoon Dehesh
May 11, 2015·Cell·Dirk M WaltherF Ulrich Hartl
Feb 26, 2016·Annual Review of Plant Biology·Dolf Weijers, Doris Wagner
May 1, 2016·Current Opinion in Plant Biology·Lucia C Strader, Yunde Zhao
Aug 20, 2016·Science·Justin W WalleySteven P Briggs
Dec 21, 2017·Proceedings of the National Academy of Sciences of the United States of America·Justin W WalleySteven P Briggs
Mar 19, 2019·Current Biology : CB·Riccardo Di MambroSabrina Sabatini

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Citations

Mar 15, 2020·Nature Plants·Mark ZanderJoseph R Ecker
Mar 27, 2021·Expert Review of Proteomics·J V Jorrin Novo
Jun 22, 2021·The Plant Journal : for Cell and Molecular Biology·Nathan D TivendaleA Harvey Millar

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