Auxin Response Factors promote organogenesis by chromatin-mediated repression of the pluripotency gene SHOOTMERISTEMLESS.

Nature Communications
Yuhee ChungDoris Wagner

Abstract

Specification of new organs from transit amplifying cells is critical for higher eukaryote development. In plants, a central stem cell pool maintained by the pluripotency factor SHOOTMERISTEMLESS (STM), is surrounded by transit amplifying cells competent to respond to auxin hormone maxima by giving rise to new organs. Auxin triggers flower initiation through Auxin Response Factor (ARF) MONOPTEROS (MP) and recruitment of chromatin remodelers to activate genes promoting floral fate. The contribution of gene repression to reproductive primordium initiation is poorly understood. Here we show that downregulation of the STM pluripotency gene promotes initiation of flowers and uncover the mechanism for STM silencing. The ARFs ETTIN (ETT) and ARF4 promote organogenesis at the reproductive shoot apex in parallel with MP via histone-deacetylation mediated transcriptional silencing of STM. ETT and ARF4 directly repress STM, while MP acts indirectly, through its target FILAMENTOUS FLOWER (FIL). Our data suggest that - as in animals- downregulation of the pluripotency program is important for organogenesis in plants.

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Citations

Dec 7, 2019·Cellular and Molecular Life Sciences : CMLS·Xiuwei Cao, Yuling Jiao
Jul 17, 2019·Cold Spring Harbor Perspectives in Biology·Yang Zhu, Doris Wagner
Oct 28, 2019·Plants·James W Satterlee, Michael J Scanlon
May 8, 2020·ELife·Carlos S Galvan-AmpudiaTeva Vernoux
Aug 23, 2019·International Journal of Molecular Sciences·Ze Hong LeeToshiro Ito
Oct 23, 2019·Biomolecules·Eduardo Mateo-BonmatíKarin Ljung
Nov 13, 2020·Plant Cell Reports·Miao Miao ZhangZhi Juan Cheng
Dec 19, 2020·Computational and Structural Biotechnology Journal·Ziyi LiuTian Hong
Dec 9, 2020·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Yahe GuoYing Wang
Dec 24, 2020·Cold Spring Harbor Perspectives in Biology·Mara CucinottaLucia Colombo
Jan 21, 2021·International Journal of Molecular Sciences·Gaojie LiHongwei Hou
May 1, 2021·International Journal of Molecular Sciences·Marina A RomanovaOlga V Voitsekhovskaja
Jun 2, 2020·Current Opinion in Plant Biology·Amy Lanctot, Jennifer L Nemhauser
Aug 28, 2021·International Journal of Molecular Sciences·Martin RasporTatjana Ćosić
Apr 28, 2021·Cold Spring Harbor Perspectives in Biology·Yanfei MaJan U Lohmann
Apr 28, 2021·Cold Spring Harbor Perspectives in Biology·Markéta Pernisová, Teva Vernoux

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Methods Mentioned

BETA
PCR
immunoprecipitation
ChIP
co-immunoprecipitation
acetylation
histone acetylation
genotyping
in vitro transcription

Software Mentioned

mVISTA
Clustal omega
BLASTn

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