Availability of adipose-derived stem cells in patients undergoing vascular surgical procedures.

The Journal of Surgical Research
Lisa J HarrisPaul J DiMuzio

Abstract

Most research evaluating adipose-derived stem cells (ASC) uses tissue obtained from young, healthy patients undergoing plastic surgical procedures. Given the propensity of other adult stem cell lines to diminish with increasing patient age and co-morbidities, we assess the availability of ASC in elderly patients undergoing vascular surgical procedures, and evaluate their acquisition of endothelial cell (EC) traits to define their potential use in vascular tissue engineering. Adipose tissue obtained by liposuction from patients undergoing vascular procedures (n = 50) was digested with collagenase and centrifuged to remove mature adipocytes. The resultant number of cells, defined as the stromal-vascular (SV) pellet, was quantified. Following a 7-d culture period and negative selection for CD31 and CD45, the resultant number of ASC was quantified. After culture in differentiating media (EMG-2), ASCs were tested for the acquisition of endothelial-specific traits (expression of CD31, realignment in shear, cord formation on Matrigel). The SV pellet contained 2.87 ± 0.34 × 10(5) cells/g fat, and the resultant number of ASCs obtained was 1.41 ± 0.18 × 10(5) cells/g fat. Flow cytometry revealed a homogeneous ASC population (>98% positiv...Continue Reading

References

Apr 1, 1989·Annals of Vascular Surgery·S K WilliamsT L Carter
Jul 1, 1997·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·A K MajorsG F Muschler
Jul 15, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·G D'IppolitoG A Howard
Dec 12, 2002·Molecular Biology of the Cell·Patricia A ZukMarc H Hedrick
May 12, 2005·Journal of Vascular Surgery·Alan DardikBauer E Sumpio
May 18, 2005·Biochemical and Biophysical Research Communications·Ying CaoRobert Chunhua Zhao
Jun 28, 2005·Trends in Biotechnology·Kevin M SalesAlexander M Seifalian
Sep 8, 2005·Current Opinion in Biotechnology·Shulamit Levenberg
Nov 2, 2005·Tissue Engineering·Gordon M RihaChangyi Chen
Jan 7, 2006·Circulation Research·Brett C IsenbergRobert T Tranquillo
Feb 17, 2006·Artificial Organs·Ralf SchäferAnton Wernig
Jul 11, 2006·The Journal of Clinical Investigation·Takashi KadowakiKazuyuki Tobe
May 6, 2008·Tissue Engineering. Part B, Reviews·Saejeong Kim, Horst von Recum
Sep 9, 2008·European Heart Journal·Gideon R HajerFrank L J Visseren
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Ning SunJoseph C Wu

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Citations

Jun 2, 2011·Cellular and Molecular Neurobiology·David ArboledaEva Sykova
Apr 26, 2012·Stem Cells and Development·Shawn P JohnsonEric D Jensen
May 11, 2012·Tissue Engineering. Part B, Reviews·Vivek K Bajpai, Stelios T Andreadis
Nov 21, 2012·Regenerative Medicine·Xiaomin HeJinghao Zheng
Aug 6, 2014·The Journal of Surgical Research·Aleksandra PolichaPaul DiMuzio
Apr 24, 2014·Journal of Clinical Medicine·Lisa B Boyette, Rocky S Tuan
Oct 5, 2013·Expert Opinion on Biological Therapy·Ching-Shwun LinTom F Lue
Jan 16, 2013·Journal of Tissue Engineering and Regenerative Medicine·Stephen McIlhennyPaul DiMuzio
Oct 28, 2015·BioMed Research International·Elizabeth A WahlThilo L Schenck
Aug 27, 2015·BioResearch Open Access·Anastasia Yu EfimenkoYelena V Parfyonova
Apr 15, 2014·Cytotherapy·Severiano Dos-Anjos VilaboaRamón Llull
Feb 7, 2012·Biomaterials·Jeffrey T Krawiec, David A Vorp
Feb 6, 2017·Scientific Reports·Alban J SmithChristopher D Wood
Feb 17, 2018·Tissue Engineering. Part a·Anh La, Robert T Tranquillo
Oct 13, 2018·Tissue Engineering. Part B, Reviews·Shigeo IchihashiPetra Mela
Jul 4, 2019·Aesthetic Plastic Surgery·Krishna S VyasKarim Bakri
Mar 31, 2011·Biotechnology and Applied Biochemistry·Mo BaguneidAlexander M Seifalian
Jul 1, 2018·International Journal of Molecular Sciences·Paola PalumboMaurizio Giuliani
Dec 17, 2016·Tissue Engineering and Regenerative Medicine·James P ChowAgneta Simionescu
Dec 9, 2020·Handchirurgie, Mikrochirurgie, plastische Chirurgie : Organ der Deutschsprachigen Arbeitsgemeinschaft für Handchirurgie : Organ der Deutschsprachigen Arbeitsgemeinschaft für Mikrochirurgie der Peripheren Nerven und Gefässe : Organ der Vereinigung der Deut·Constanze KuhlmannPaul Severin Wiggenhauser
Dec 29, 2020·Journal of Neuropathology and Experimental Neurology·Saurav Bhowmick, P M Abdul-Muneer
Feb 6, 2018·Acta Biomaterialia·Jhilmil Dhulekar, Agneta Simionescu

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