Avian haemosporidian parasites (Haemosporida): A comparative analysis of different polymerase chain reaction assays in detection of mixed infections

Experimental Parasitology
Rasa BernotienėGediminas Valkiūnas

Abstract

Mixed infections of different species and genetic lineages of haemosporidian parasites (Haemosporida) predominate in wildlife, and such infections are particularly virulent. However, currently used polymerase chain reaction (PCR)-based detection methods often do not read mixed infections. Sensitivity of different PCR assays in detection of mixed infections has been insufficiently tested, but this knowledge is essential in studies addressing parasite diversity in wildlife. Here, we applied five different PCR assays, which are broadly used in wildlife avian haemosporidian research, and compared their sensitivity in detection of experimentally designed mixed infections of Haemoproteus and Plasmodium parasites. Three of these PCR assays use primer sets that amplify fragments of cytochrome b gene (cyt b), one of cytochrome oxidase subunit I (COI) gene, and one target apicoplast genome. We collected blood from wild-caught birds and, using microscopic and PCR-based methods applied in parallel, identified single infections of ten haemosporidian species with similar parasitemia. Then, we prepared 15 experimental mixes of different haemosporidian parasites, which often are present simultaneously in wild birds. Similar concentration of to...Continue Reading

References

Apr 29, 1998·International Journal for Parasitology·T N Petney, R H Andrews
May 25, 2002·Proceedings. Biological Sciences·Robert E Ricklefs, Sylvia M Fallon
Aug 29, 2002·The Journal of Parasitology·F Alexander RichardThomas B Smith
Feb 10, 2004·Journal of Clinical Microbiology·Hiroshi YamasakiAkira Ito
Sep 3, 2004·Evolution; International Journal of Organic Evolution·Staffan BenschOlof Hellgren
Sep 11, 2004·The Journal of Parasitology·Olof HellgrenStaffan Bensch
Jun 22, 2005·Trends in Parasitology·Gediminas ValkiūnasMichael A Peirce
Aug 20, 2005·The Journal of Parasitology·Jon S Beadell, Robert C Fleischer
May 9, 2008·Journal of Evolutionary Biology·A MarzalF De Lope
Jun 26, 2008·The Journal of Parasitology·Gediminas ValkiunasStaffan Bensch
Oct 29, 2009·The Journal of Parasitology·Claire LoiseauRavinder N M Sehgal
Dec 17, 2009·The Journal of Parasitology·Vaidas PalinauskasStaffan Bensch
May 1, 1997·Trends in Ecology & Evolution·G SorciT Boulinier
Jul 24, 2012·Parasitology International·Adriana Silva-IturrizaRalph Tiedemann
Dec 18, 2013·Parasitology Research·Gediminas ValkiūnasTatjana A Iezhova
Dec 3, 2014·Experimental Parasitology·Dimitar DimitrovGediminas Valkiūnas
Mar 15, 2015·International Journal for Parasitology·Juliane SchaerSusan L Perkins
Jul 19, 2015·Parasites & Vectors·Jeffrey A BellVasyl V Tkach

❮ Previous
Next ❯

Citations

Mar 20, 2016·Experimental Parasitology·Radovan VáclavFrancisco Valera
Jul 17, 2016·Acta Tropica·Gediminas ValkiūnasRavinder N M Sehgal
Aug 17, 2016·Proceedings of the National Academy of Sciences of the United States of America·Gregory KaradjianLinda Duval
Sep 28, 2016·Parasitology Research·Eszter SzöllősiJános Török
Dec 27, 2016·Parasitology Research·Martin Ward, Giovanni Benelli
Aug 14, 2019·The Journal of Animal Ecology·Spencer C GalenSusan L Perkins
Feb 28, 2020·Parasites & Vectors·Carolina R F ChagasGediminas Valkiūnas
Jun 10, 2017·Malaria Journal·Josué Martínez-de la PuenteJordi Figuerola
Dec 29, 2017·Parasitology Research·Jamie D SmithMaarten J Vonhof
Jul 28, 2019·Ecology and Evolution·Meghann B HumphriesRobert E Ricklefs
May 31, 2018·Parasitology Research·Jenny S CarlsonAnthony J Cornel
May 31, 2018·Malaria Journal·Gediminas Valkiūnas, Tatjana A Iezhova
May 4, 2018·Malaria Journal·Gediminas ValkiūnasTatjana A Iezhova
Mar 21, 2020·Frontiers in Cellular and Infection Microbiology·Ricardo CorreaCarmenza Spadafora
Jun 19, 2019·International Journal for Parasitology. Parasites and Wildlife·Pornchai PornpanomChaleow Salakij
Oct 13, 2020·International Journal for Parasitology·S ReisC Loiseau
Jan 9, 2019·International Journal for Parasitology. Parasites and Wildlife·Mamohale E ChaisiEssa Suleman
Mar 9, 2021·Veterinary Parasitology : Regional Studies and Reports·Milan ThorelAntoine Leclerc
Jun 3, 2021·Pathogens·Vajiheh GhaemitalabMansour Aliabadian
Aug 12, 2021·International Journal for Parasitology. Parasites and Wildlife·Mizue InumaruAlfonso Marzal
Aug 26, 2021·Ecology and Evolution·Vincent BourretClaire Loiseau

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.