Avian influenza viral nucleocapsid and hemagglutinin proteins induce chicken CD8+ memory T lymphocytes.

Virology
Shailbala SinghEllen W Collisson

Abstract

The avian influenza viruses (AIVs) can be highly contagious to poultry and a zoonotic threat to humans. Since the memory CD8(+) T lymphocyte responses in chickens to AIV proteins have not been defined, these responses to H5N9 AIV hemagglutinin (HA) and nucleocapsid (NP) proteins were evaluated by ex vivo stimulation with virus infected non-professional antigen presenting cells. Secretion of IFNgamma by activated T lymphocytes was evaluated through macrophage induction of nitric oxide. AIV specific, MHC-I restricted memory CD8(+) T lymphocyte responses to NP and HA were observed 3 to 9 weeks post-inoculation (p.i.). The responses specific to NP were greater than those to HA with maximum responses being observed at 5 weeks p.i. followed by a decline to weakly detectable levels by 9 weeks p.i. The cross-reaction of T lymphocytes to a heterologous H7N2 AIV strain demonstrated their ability to respond to a broader range of AIV.

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Citations

Nov 28, 2012·Developmental and Comparative Immunology·Ghida R BanatEllen W Collisson
May 3, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Seyed Davoud JazayeriAbdul Rahman Omar
Apr 20, 2012·Comparative Immunology, Microbiology and Infectious Diseases·Seyed Davoud JazayeriAbdul Rahman Omar
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Dec 23, 2016·Proceedings. Biological Sciences·Sarah C HillOliver G Pybus
Jun 29, 2013·Journal of Zoo and Wildlife Medicine : Official Publication of the American Association of Zoo Veterinarians·Yvonne DrechslerEllen W Collisson

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BETA
ELISA
flow cytometry
PCR
density gradient centrifugation
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