Avian malaria co-infections confound infectivity and vector competence assays of Plasmodium homopolare

Parasitology Research
Jenny S CarlsonAnthony J Cornel

Abstract

Currently, there are very few studies of avian malaria that investigate relationships among the host-vector-parasite triad concomitantly. In the current study, we experimentally measured the vector competence of several Culex mosquitoes for a newly described avian malaria parasite, Plasmodium homopolare. Song sparrow (Melospiza melodia) blood infected with a low P. homopolare parasitemia was inoculated into a naïve domestic canary (Serinus canaria forma domestica). Within 5 to 10 days post infection (dpi), the canary unexpectedly developed a simultaneous high parasitemic infection of Plasmodium cathemerium (Pcat6) and a low parasitemic infection of P. homopolare, both of which were detected in blood smears. During this infection period, PCR detected Pcat6, but not P. homopolare in the canary. Between 10 and 60 dpi, Pcat6 blood stages were no longer visible and PCR no longer amplified Pcat6 parasite DNA from canary blood. However, P. homopolare blood stages remained visible, albeit still at very low parasitemias, and PCR was able to amplify P. homopolare DNA. This pattern of mixed Pcat6 and P. homopolare infection was repeated in three secondary infected canaries that were injected with blood from the first infected canary. Mosq...Continue Reading

References

Jul 1, 1978·The American Journal of Tropical Medicine and Hygiene·M J Klowden, A O Lea
Mar 1, 1990·Transactions of the Royal Society of Tropical Medicine and Hygiene·R RosenbergR Burge
Apr 1, 1994·The American Journal of Tropical Medicine and Hygiene·N SuwanabunR Rosenberg
Nov 1, 1996·Transactions of the Royal Society of Tropical Medicine and Hygiene·K MaitlandD K Bowden
May 23, 2001·Trends in Ecology & Evolution·H McCallumJ Hone
Jun 18, 2002·Proceedings. Biological Sciences·H M Ferguson, A F Read
Jun 15, 2004·Trends in Parasitology·Georges Snounou, Nicolas J White
Nov 26, 2005·Trends in Parasitology·Christophe Boëte, Richard E L Paul
Apr 4, 2008·Molecular Biology and Evolution·Deirdre A JoyXin-zhuan Su
Apr 22, 2008·Molecular Ecology·Andrea B GagerEldredge Bermingham
Jun 26, 2008·The Journal of Parasitology·Gediminas ValkiunasStaffan Bensch
Jul 11, 2009·BMC Evolutionary Biology·Louis LambrechtsThomas W Scott
Feb 11, 2012·Annals of the New York Academy of Sciences·Dennis A LapointeMichael D Samuel
Jan 23, 2013·Experimental Parasitology·Rita KazlauskienėGediminas Valkiūnas
Apr 19, 2013·Proceedings. Biological Sciences·Matthew C I MedeirosRobert E Ricklefs
Aug 8, 2013·Journal of the American Mosquito Control Association·Jonathan C Winchester, Durrell D Kapan
Aug 21, 2013·The American Journal of Tropical Medicine and Hygiene·Linda KotheraHarry M Savage
Dec 3, 2014·Experimental Parasitology·Dimitar DimitrovGediminas Valkiūnas
Jul 15, 2015·Parasites & Vectors·Jenny S CarlsonAnthony J Cornel
Oct 26, 2016·The Journal of Animal Ecology·Nicholas J ClarkSonya M Clegg

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Datasets Mentioned

BETA
KJ482708
KJ620779
KJ620781
and

Methods Mentioned

BETA
PCR
dissections
laser capture microdissections

Software Mentioned

R
R Core Team

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