Avian reovirus σA-modulated suppression of lactate dehydrogenase and upregulation of glutaminolysis and the mTOC1/eIF4E/HIF-1α pathway to enhance glycolysis and the TCA cycle for virus replication

Cellular Microbiology
Pei-I ChiHung-Jen Liu

Abstract

Adenosine triphosphate (ATP) is an energy source for many types of viruses for facilitating virus replication. This is the first report to demonstrate that the structural protein σA of avian reovirus (ARV) functions as an activator of cellular energy. Three cellular factors, isocitrate dehydrogenase 3 subunit beta (IDH3B), lactate dehydrogenase A (LDHA), and vacuolar-type H+-ATPase (vATPase) co-immunoprecipitated with ARV σA and were identified by 2D-LC/MS/MS. ARV enhances glycolytic flux through upregulation of glycolytic enzymes. Increased ATP levels in both ARV-infected and σA-transfected cells were observed by a fluorescence resonance energy transfer-based genetically encoded indicator, Ateams. Furthermore, σA upregulates IDH3B and glutamate dehydrogenase (GDH) to promote glutaminolysis, activating HIF-1α. Both HIF-1α level and viral yield in IDH3B-depleted and glutamine-deprived cells, and inhibition of glutaminolysis was significantly reduced. The σAR155/273A mutant loses its ability to enter the nucleolus, impairing its ability to regulate glycolysis. In addition, we have identified the conserved untranslated regions (UTR) of the 5'- and 3'-termini of the ARV genome segments that are required for viral protein synthesis ...Continue Reading

References

Jun 6, 1995·Proceedings of the National Academy of Sciences of the United States of America·G L WangG L Semenza
Sep 15, 1998·Methods : a Companion to Methods in Enzymology·B L JacobsT Brandt
Sep 21, 2002·Molecular and Cellular Biology·Christine C HudsonRobert T Abraham
May 29, 2003·The Journal of General Virology·Claudia González-LópezJavier Benavente
Dec 23, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Eun-Joung MoonKyu-Won Kim
Jun 1, 2004·Molecular and Cellular Biology·Naohiro WakisakaJoseph S Pagano
Jan 14, 2005·Molecular and Cellular Biochemistry·Tatiana El-BachaAndrea T Da Poian
Apr 14, 2005·Journal of Virology·Cary A MoodyJohn W Sixbey
Apr 26, 2005·Journal of Virological Methods·Andrew LutzAndrew Pekosz
Oct 5, 2006·Virus Research·Javier Benavente, Jose Martínez-Costas
Dec 19, 2006·PLoS Pathogens·Joshua MungerJoshua D Rabinowitz
Feb 20, 2007·Circulation Research·Mhairi C Towler, D Grahame Hardie
Sep 19, 2008·Journal of Virology·Pablo Guardado-CalvoMark J van Raaij
Jun 13, 2009·Apoptosis : an International Journal on Programmed Cell Death·Chi-Hung LinHung-Jeng Liu
Sep 2, 2009·Proceedings of the National Academy of Sciences of the United States of America·Hiromi ImamuraHiroyuki Noji
Sep 4, 2009·Journal of Biomedical Science·Chia-Wei ChangShih-Yen Lo
Nov 27, 2009·Journal of Virology·Jeremy W ChambersJames C Alwine
Jun 25, 2010·Theoretical Biology & Medical Modelling·Stephen Rothman
May 1, 2012·Biochimica Et Biophysica Acta·Juliana L AbrantesThiago Moreno L Souza
Jul 23, 2013·Oncotarget·Di ZhaoKu-Liang Guan
Jan 5, 2014·The Journal of Cell Biology·Charles Betz, Michael N Hall
Jan 7, 2014·Journal of Virology·Christophe RamièreOlivier Diaz
Jan 23, 2014·Proceedings of the National Academy of Sciences of the United States of America·Yongjun YuJames C Alwine
Feb 7, 2014·Journal of Virology·Krystal A FontaineMichael Lagunoff
Feb 11, 2014·Cancer Letters·Nicholas WongDamu Tang
Mar 22, 2014·Nature Reviews. Molecular Cell Biology·Keisuke Ito, Toshio Suda

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Citations

Feb 14, 2020·The Journal of Veterinary Medical Science·Karla Cristine C DoysabasEiichi Hondo
May 11, 2019·Nature Communications·Nidhi Kedia-Mehta, David K Finlay
Feb 27, 2021·Journal of Theoretical Biology·Chong YuJin Wang
Sep 30, 2021·Annual Review of Virology·Khyati GirdharEmrah Altindis

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