Avian sarcoma virus and human immunodeficiency virus, type 1 use different subsets of ESCRT proteins to facilitate the budding process.

The Journal of Biological Chemistry
Andrew PinceticJonathan Leis

Abstract

Members of the Nedd4 family of E3 ubiquitin ligases bind the L domain in avian sarcoma virus (ASV) Gag and facilitate viral particle release. Translational fusion of ASV Gag with an L domain deletion (Deltap2b) to proteins that comprise ESCRT-I, -II, and -III (the endocytic sorting complexes required for transport) rescued both Gag ubiquitination and particle release from cells. The ESCRT-I factors Vps37C or Tsg101 were more effective in rescue of Gag/Deltap2b budding than the ESCRT-II factor Eap20 or the ESCRT-III component CHMP6. Thus ESCRT components can substitute for Nedd4 family members in ASV Gag release. Unlike wild type, ASV Gag/Deltap2b -ESCRT chimeras failed to co-immunoprecipitate with co-expressed hemagglutinin-tagged Nedd4, indicating that Nedd4 was not stably associated with these Gag fusions. Release of the Gag-ESCRT-I or -II fusions was inhibited by a dominant negative mutant of Vps4 ATPase similar to wild type ASV Gag. In contrast to ASV Gag, HIV-1 Gag containing an L domain inactivating mutation (P7L) was efficiently rescued by fusion to a component of ESCRT-III (Chmp6) but not ESCRT-II (Eap20). Depletion of the endogenous pool of Eap20 (ESCRT-II) had little effect on HIV-1 Gag release but blocked ASV Gag rel...Continue Reading

References

Apr 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·H G GöttlingerW A Haseltine
Jun 1, 1990·Virology·D PuttermanV M Vogt
Nov 23, 2000·Proceedings of the National Academy of Sciences of the United States of America·U SchubertJ W Yewdell
Nov 23, 2000·Proceedings of the National Academy of Sciences of the United States of America·A PatnaikJ W Wills
Dec 9, 2000·Virology·D E OttU Schubert
Jun 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·L VerPlankC A Carter
Sep 20, 2001·Proceedings of the National Academy of Sciences of the United States of America·A KikonyogoJ Leis
Mar 29, 2003·Journal of Virology·Juan Martin-SerranoPaul D Bieniasz
Aug 2, 2003·Current Opinion in Cell Biology·Camilla RaiborgHarald Stenmark
Aug 6, 2003·The Journal of Cell Biology·Owen PornillosWesley I Sundquist
Sep 25, 2003·Cell·Bettina StrackHeinrich G Göttlinger
Sep 25, 2003·Cell·Uta K von SchwedlerWesley I Sundquist
Dec 25, 2003·Journal of Virology·Miranda Shehu-XhilagaEric O Freed
Jan 28, 2004·Cell Structure and Function·Kyohei Umebayashi
Apr 9, 2004·Nature Reviews. Molecular Cell Biology·Jean Gruenberg, Harald Stenmark
May 14, 2004·Journal of Virology·Juan Martin-SerranoPaul D Bieniasz
Jun 26, 2004·The Journal of Biological Chemistry·Melissa D StuchellWesley I Sundquist
Sep 16, 2004·Journal of Virology·Jared L SpidelJohn W Wills
Dec 1, 2004·Traffic·Markus Babst
Dec 30, 2004·The Journal of Cell Biology·Juan Martin-SerranoPaul D Bieniasz
Mar 15, 2006·The Journal of Cell Biology·Amy M BoothStephen J Gould
May 13, 2006·Annual Review of Biophysics and Biomolecular Structure·James H Hurley, Scott D Emr
Jun 9, 2006·The Journal of Biological Chemistry·Monica Agromayor, Juan Martin-Serrano
Jun 20, 2006·Current Opinion in Cell Biology·Matthew Robert Geoffrey RussellGreg Odorizzi
Sep 16, 2006·Journal of Virology·Charles LangelierWesley I Sundquist
Dec 13, 2006·Cell Structure and Function·Masanao KyuumaNobuyuki Tanaka
Dec 14, 2007·Molecular Biology of the Cell·Bethan McDonald, Juan Martin-Serrano

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Citations

May 6, 2011·Journal of Virology·G N MedinaC A Carter
Jan 7, 2011·Journal of Virology·Mark A YondolaPeter Palese
May 6, 2011·Journal of Virology·Zhizhou KuangJonathan Leis
Oct 30, 2009·Advances in Virology·Andrew Pincetic, Jonathan Leis
Mar 13, 2014·PloS One·Jens T Stieler, Reinhild Prange
Jun 15, 2011·Viruses·Mark Yondola, Carol Carter
Mar 27, 2013·Annual Review of Biochemistry·John McCulloughWesley I Sundquist
Aug 31, 2011·Journal of Molecular Biology·Lorna S EhrlichCarol A Carter
Dec 31, 2008·Antiviral Research·Ronald N Harty
Sep 18, 2012·Biology of the Cell·Bashar GhoujalAndrew J Mouland
Jul 5, 2011·Traffic·S Brookhart Shields, Robert C Piper
Oct 16, 2009·The Journal of General Virology·Lynsey CorlessMark Harris
Apr 21, 2016·Viruses·Christina Bartusch, Reinhild Prange
Mar 14, 2007·PLoS Computational Biology·Aleksejs KontijevskisJarl E S Wikberg
Jul 5, 2012·Molecular Biology International·Lorna S Ehrlich, Carol A Carter
Aug 5, 2017·Journal of Virology·Ziying HanRonald N Harty
Mar 13, 2010·Biology of the Cell·Ingrid RoxrudLene Malerød
Jun 18, 2017·Biochemical Society Transactions·Edward J Scourfield, Juan Martin-Serrano
Jan 11, 2020·Cellular Microbiology·Bo MengAndrew M L Lever
Sep 20, 2020·Viruses·Shilpa GuptaSaveez Saffarian
Jan 16, 2009·Biochemical Society Transactions·J Paul LuzioNicholas A Bright
Sep 15, 2019·The Journal of Biological Chemistry·Huxley K HoffmanSchuyler B van Engelenburg
Oct 1, 2020·The Journal of Biological Chemistry·Birgit MeusserFriedrich C Luft
Jan 18, 2021·The Journal of Biological Chemistry·Birgit MeusserFriedrich C Luft
Mar 7, 2021·Cells·Arianna CalistriCristina Parolin
Aug 5, 2016·Journal of Virology·Ziying HanRonald N Harty
Aug 28, 2021·International Journal of Molecular Sciences·Yichen JuLiying Zhang

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