PMID: 6390435Dec 1, 1984Paper

Avidin-biotin affinity chromatography: application to the isolation of human placental insulin receptor

Proceedings of the National Academy of Sciences of the United States of America
F M FinnK Hofmann

Abstract

The ligand N alpha, B1-(6-biotinylamido)hexanoyl-insulin was attached noncovalently to Sepharose 4B immobilized succinoylavidin to form an insulin-affinity resin. This resin was used to isolate highly purified insulin receptor from human placental tissue by a four step process involving (i) preparation of a crude membrane fraction, (ii) solubilization with Triton X-100, (iii) wheat germ agglutinin purification, and (iv) insulin-affinity chromatography. NaDodSO4/PAGE of the purified 125I-labeled receptor under nonreducing conditions showed the presence of a major component with an approximate molecular weight of 350,000 and a minor component with a molecular weight of approximately equal to 166,000. Based on the assumption that the degree of labeling is comparable in both components, the material corresponding to the Mr 350,000 peak represents approximately equal to 94% of the receptor preparation as determined by scanning the autoradiograms. The specific insulin binding capacity of the preparation is 18 +/- 6 micrograms of 125I-labeled insulin per mg of protein as determined by the polyethylene glycol assay and analyzed by Scatchard plot. Insulin binding activity was stable at 4 degrees C and pH 7.6 for at least 12 weeks but wa...Continue Reading

References

Oct 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·K Hofmann, Y Kiso
Jul 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·K HofmannH Zahn
Aug 28, 1979·Biochimica Et Biophysica Acta·P F Williams, J R Turtle
Aug 8, 1977·Biochemical and Biophysical Research Communications·S JacobsP Cuatrecasas
May 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·P Cuatrecasas
Mar 1, 1973·Archives of Biochemistry and Biophysics·P BöhlenS Udenfriend
Nov 1, 1965·Bulletin of the Chemical Society of Japan·S Sakakibara, N Inukai
Jan 1, 1981·Endocrine Reviews·S Jacobs, P Cuatrecasas
Jun 5, 1984·Biochemistry·K HofmannP K Mishra
Jun 5, 1984·Biochemistry·F M FinnK Hofmann
Mar 2, 1982·Biochemistry·K HofmannF M Finn
Oct 1, 1964·Proceedings of the Society for Experimental Biology and Medicine·R WEI, L D WRIGHT

❮ Previous
Next ❯

Citations

Jun 1, 1989·Journal of Virological Methods·E KorecI Hlozánek
Nov 19, 1986·Biochimica Et Biophysica Acta·V R MuzykantovV N Smirnov
Mar 6, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·J Turková
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·K D RidgeF M Finn
Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·F M FinnK Hofmann
Nov 15, 1985·Biochemical and Biophysical Research Communications·J D Newman, L C Harrison
Sep 1, 1985·Analytical Biochemistry·E A BayerM Wilchek
Jun 13, 1986·Biochemical and Biophysical Research Communications·P Maly, C Lüthi
Jun 1, 1991·Analytical Biochemistry·I Pieri, D Barritault
May 15, 1988·Analytical Biochemistry·M Wilchek, E A Bayer
Aug 14, 1986·Biochemical and Biophysical Research Communications·P Maly, C Lüthi
Nov 15, 2002·Biochimica Et Biophysica Acta·Essam MetwallyRonald Mathison
Jan 1, 1995·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·D H KimA A García
Mar 1, 1993·The Korean Journal of Parasitology·N S WooY K Choi
Dec 29, 2000·American Journal of Veterinary Research·R Paxton, L X Ye
Sep 21, 2019·Biological Chemistry·Lisa Maria KöglerAnnette G Beck-Sickinger
Sep 1, 1989·International Journal of Peptide and Protein Research·R SeyerB Castro
Nov 26, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lixin LiuQun Wei
Feb 1, 1987·American Journal of Reproductive Immunology and Microbiology : AJRIM·I C Kim, C L Sabourin
Mar 1, 1989·Biological Chemistry Hoppe-Seyler·F WedekindD Brandenburg

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.