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Avoidance of strongly chaotropic eluents for immunoaffinity chromatography by chemical modification of immobilized ligand

Biochimica Et Biophysica Acta

Jan 20, 1976

Richard Andrew MurphyDavid W Elmore

PMID: 2298

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Abstract

The need for chaotropic eluents in immunoaffinity chromatography is a consequence of the high affinities of antibodies towards their antigens. This affinity is decreased and elution of antiglucagon antibodies from a column of immobilized glucagon can be achieved under mild conditions wh...read more

Mentioned in this Paper

Hydrogen Peroxide
Ligands
Oxidation-Reduction
Immunoaffinity Chromatography
Iodopeptides
Binding Sites, Antibody
Glucagon
Metazoa
Chinchilla Rabbits
Glucagon (rDNA)
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  • Avoidance of strongly chaotropic eluents for immunoaffinity chromatography by chemical modification of immobilized ligand

    Biochimica Et Biophysica Acta

    Jan 20, 1976

    Richard Andrew MurphyDavid W Elmore

    PMID: 2298

    DOI:

    Abstract

    The need for chaotropic eluents in immunoaffinity chromatography is a consequence of the high affinities of antibodies towards their antigens. This affinity is decreased and elution of antiglucagon antibodies from a column of immobilized glucagon can be achieved under mild conditions wh...read more

    Mentioned in this Paper

    Hydrogen Peroxide
    Ligands
    Oxidation-Reduction
    Immunoaffinity Chromatography
    Iodopeptides

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