Aug 3, 2015

Avoiding accuracy-limiting pitfalls in the study of protein-ligand interactions with isothermal titration calorimetry

BioRxiv : the Preprint Server for Biology
Sarah E. BoyceJohn D Chodera

Abstract

Isothermal titration calorimetry (ITC) can yield precise (±3%) estimates of the thermodynamic parameters describing biomolecular association (affinity, enthalpy, and entropy), making it an indispensable tool for biochemistry and drug discovery. Surprisingly, interlaboratory comparisons suggest that errors of ∼20% are common and widely underreported. Here, we show how to reduce precision- and accuracy-limiting errors while obtaining good estimates and minimizing material and time consumed by an experiment. We provide a simple spreadsheet that allows practitioners to identify precision-limiting operations during protocol design, track precision during the experiment, and propagate error to yield realistic final uncertainties.

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Mentioned in this Paper

Study
Calorimetry
Ligands Activity
Titration Method
Ligands
Practitioner
Research Study

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