AWRK6, A Synthetic Cationic Peptide Derived from Antimicrobial Peptide Dybowskin-2CDYa, Inhibits Lipopolysaccharide-Induced Inflammatory Response

International Journal of Molecular Sciences
Qiuyu WangDianbao Zhang

Abstract

Lipopolysaccharides (LPS) are major outer membrane components of Gram-negative bacteria and produce strong inflammatory responses in animals. Most antibiotics have shown little clinical anti-endotoxin activity while some antimicrobial peptides have proved to be effective in blocking LPS. Here, the anti-LPS activity of the synthetic peptide AWRK6, which is derived from antimicrobial peptide dybowskin-2CDYa, has been investigated in vitro and in vivo. The positively charged α-helical AWRK6 was found to be effective in blocking the binding of LBP (LPS binding protein) with LPS in vitro using ELISA. In a murine endotoxemia model, AWRK6 offered satisfactory protection efficiency against endotoxemia death, and the serum levels of LPS, IL-1β, IL-6, and TNF-α were found to be attenuated using ELISA. Further, histopathological analysis suggested that AWRK6 could improve the healing of liver and lung injury in endotoxemia mice. The results of real-time PCR and Western blotting showed that AWRK6 significantly reversed LPS-induced TLR4 overexpression and IκB depression, as well as the enhanced IκB phosphorylation. Additionally, AWRK6 did not produce any significant toxicity in vivo and in vitro. In summary, AWRK6 showed efficacious protect...Continue Reading

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Citations

Jul 24, 2018·Journal of Biomaterials Science. Polymer Edition·Guoli YangLingling Dong
Oct 12, 2018·International Journal of Molecular Sciences·Qiuyu WangDianbao Zhang
Sep 13, 2018·International Journal of Molecular Sciences·Lili JinDianbao Zhang
Apr 13, 2021·The Journal of Biological Chemistry·Gairika GhoshRanjan Sen

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Datasets Mentioned

BETA
ACF08009.1

Methods Mentioned

BETA
enzyme-linked immunosorbent assay
PCR
ELISA
light microscopy
protein assay

Software Mentioned

GraphPad
GraphPad Prism
ANTHEPROT

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