PMID: 8591018Dec 1, 1995Paper

Axenization of Entamoeba histolytica, a Korean strain YS-27

The Korean Journal of Parasitology
J K ChangC T Soh

Abstract

In the present communication, YS-27, a Korean strain of Entamoeba histolytica is described for the isolation and establishment of axenic cultivation. E. histolytica, designated as strain "YS-27", was isolated from the pus of a hepatic abscess obtained from a 72 year old inpatient of August 10, 1969. Specimens, were obtained by needle aspiration, inoculated immediately and weekly cultured in a modified diphasic medium at 37 degrees C. Strain YS-27 had been maintained for more than 15 years by weekly subculture until February, 1985. These cultures were transferred to a monoxenic TTY-SB medium seeded with a trypanosomatid of the genus Crithidia. Penicillin G, 2 to 10 x 10(3) International units and Streptomycin, 2 to 10 mg per 100 ml, were added to the cultures to eliminate the bacteria. After more than one year later, these two organisms were well maintained by transfer every 3 or 4 days until January, 1986 at 37 degrees C in TTY-SB medium in the absence of other microorganisms. These monoxenic cultures were then transferred to TYI-S-33 medium. Strain YS-27 alone had not been growing at the time of transfer, but when overlaid with Crithidia at intervals of 3 to 4 days, strain YS-27 propagated well. The Crithidia died out several ...Continue Reading

References

Jan 1, 1978·Transactions of the Royal Society of Tropical Medicine and Hygiene·L S DiamondC C Cunnick
Aug 4, 1961·Science·L S DIAMOND
May 1, 1960·The Journal of Infectious Diseases·C T PAN

❮ Previous
Next ❯

Citations

May 30, 2001·Molecular and Biochemical Parasitology·S J ParkT S Yong
Apr 16, 2016·Parasitology International·Sharmina DeloerShinjiro Hamano
Oct 23, 1997·The Korean Journal of Parasitology·K I ImM S La
Apr 3, 1999·The Korean Journal of Parasitology·S J ParkC T Soh
Mar 1, 1996·The Korean Journal of Parasitology·S C ChoeS T Hong
Dec 1, 1996·The Korean Journal of Parasitology·M Lee, S T Hong

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Amoebiasis

Amoebiasis, infection by the protozoan parasite Entamoeba histolytica, remains a global health problem, despite the availability of effective treatment. Here is the latest research.