PMID: 8941935Dec 1, 1996Paper

Axial distribution of band 3-positive intercalated cells in the collecting duct of control and ammonium chloride-loaded rabbits

Kidney International. Supplement
Jill W VerlanderC C Tisher

Abstract

Recent studies have suggested that less than 10% of intercalated cells in the rabbit outer cortical collecting duct (CCD) [1, 2] and less than 3% in the connecting segment (CNT) [3] are identifiable by functional criteria as acid-secreting (type A or alpha) intercalated cells. Other studies, using peanut lectin-binding and the absence of apical endocytic activity to identify bicarbonate-secreting (type B or beta) intercalated cells, have suggested that acid-loading increases the percentage of alpha intercalated cells in the CCD. Because our preliminary observations of band 3 immunoreactivity suggest that the percentages of alpha intercalated cells in the rabbit outer CCD and the CNT are underestimated by physiologic studies and are not altered by chronic acid-loading, we quantified the percentage of alpha intercalated cells in various segments of the collecting duct using light microscopic immunohistochemistry in kidneys of rabbits receiving tap water (control) or 75 mM NH4Cl for 12 days plus 8 daily gavages of 2 to 6 mEq NH4Cl/kg body wt. Mean urine pH values were 5.96 in acid-loaded animals versus 8.47 in controls. Kidneys were preserved by in vivo perfusion with periodatelysine-paraformaldehyde fixation and processed for imm...Continue Reading

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