Axin, a negative regulator of the wnt signaling pathway, directly interacts with adenomatous polyposis coli and regulates the stabilization of beta-catenin.

The Journal of Biological Chemistry
S KishidaA Kikuchi

Abstract

The regulators of G protein signaling (RGS) domain of Axin, a negative regulator of the Wnt signaling pathway, made a complex with full-length adenomatous polyposis coli (APC) in COS, 293, and L cells but not with truncated APC in SW480 or DLD-1 cells. The RGS domain directly interacted with the region containing the 20-amino acid repeats but not with that containing the 15-amino acid repeats of APC, although both regions are known to bind to beta-catenin. In the region containing seven 20-amino acid repeats, the region containing the latter five repeats bound to the RGS domain of Axin. Axin and beta-catenin simultaneously interacted with APC. Furthermore, Axin stimulated the degradation of beta-catenin in COS cells. Taken together with our recent observations that Axin directly interacts with glycogen synthase kinase-3beta (GSK-3beta) and beta-catenin and that it promotes GSK-3beta-dependent phosphorylation of beta-catenin, these results suggest that Axin, APC, GSK-3beta, and beta-catenin make a tetrameric complex, resulting in the regulation of the stabilization of beta-catenin.

References

Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·S MunemitsuP Polakis
Dec 10, 1993·Science·B RubinfeldP Polakis
Jan 5, 1996·The Journal of Biological Chemistry·A Kikuchi, L T Williams
Feb 14, 1997·The Journal of Biological Chemistry·H G Dohlman, J Thorner
Jun 7, 1997·Biochimica Et Biophysica Acta·P Polakis
Jul 1, 1997·The EMBO Journal·H AberleR Kemler
Feb 1, 1949·The Journal of Experimental Zoology·S GLUECKSOHN-SCHOENHEIMER

❮ Previous
Next ❯

Citations

Dec 2, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·P J Morin
Sep 14, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J Kitajewski, D Sassoon
Dec 18, 2001·Genes, Chromosomes & Cancer·Yosuke ShimizuToshimasa Asahara
May 17, 2002·The Journal of Pathology·Robert G HardyD Scott A Sanders
Dec 3, 1999·Biochemical and Biophysical Research Communications·E h JhoF Costantini
Feb 19, 2000·Biochemical and Biophysical Research Communications·A Kikuchi
Jun 29, 2000·Biochemical and Biophysical Research Communications·S Y NeoS C Lin
Jun 19, 2007·Medical Molecular Morphology·Takao SendaAtsushi Shimomura
Jan 21, 2009·Cancer Metastasis Reviews·Otto SchmalhoferThomas Brabletz
Nov 6, 2010·Medical Oncology·Nighat ParveenSyed Mudassar
Sep 26, 2009·Cancer Microenvironment : Official Journal of the International Cancer Microenvironment Society·Pawan KalerLidija Klampfer
Nov 30, 2012·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Lian-He YangEn-Hua Wang
Aug 27, 1999·Trends in Pharmacological Sciences·J R Hepler
Apr 17, 2002·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Roy M BremnesWilbur A Franklin
Aug 31, 2001·Progress in Neurobiology·C A Grimes, R S Jope
Jun 5, 2003·Biochimica Et Biophysica Acta·Rachel H GilesHans Clevers
Jan 5, 2000·Cellular Signalling·A Kikuchi
Nov 18, 1998·Current Opinion in Cell Biology·A Ben-Ze'ev, B Geiger
Apr 21, 1999·Current Opinion in Cell Biology·Q Eastman, R Grosschedl
Sep 12, 2000·Current Opinion in Genetics & Development·L Kim, A R Kimmel
Mar 11, 1999·Current Opinion in Genetics & Development·P Polakis
Aug 17, 1999·Current Opinion in Genetics & Development·S Y Sokol
May 4, 1999·Trends in Cell Biology·L De Vries, M Gist Farquhar
Nov 5, 1999·Trends in Biochemical Sciences·B ZhengM Gist Farquhar
Aug 26, 2000·Cytokine & Growth Factor Reviews·T Akiyama
Jan 27, 2000·Cytokine & Growth Factor Reviews·A Kikuchi
Feb 3, 2005·Cell Research·Roel Nusse
Sep 21, 2001·The Biochemical Journal·S Frame, P Cohen
Jun 13, 2002·European Journal of Clinical Investigation·Irma M Oving, Hans C Clevers
Oct 20, 1999·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·F HamadaT Akiyama
Oct 21, 2000·Development, Growth & Differentiation·A FukuiM Asashima
Nov 8, 2002·Journal of Neurochemistry·William H StoothoffGail V W Johnson
May 13, 2009·The Journal of Biological Chemistry·Sajid RashidMarta Miaczynska
Oct 8, 2008·The Journal of Biological Chemistry·Zhenghong LinYe-Guang Chen
Dec 3, 2008·The Journal of Biological Chemistry·Lilia TopolYingzi Yang
May 13, 2003·The Proceedings of the Nutrition Society·John C MathersUNKNOWN Concerted Action Polyp Prevention (CAPP) 1 Study
Jan 14, 2004·The Journal of Experimental Medicine·Monica CobasFreddy Radtke
Jan 15, 2005·Thyroid : Official Journal of the American Thyroid Association·Takeshi KuriharaToshimasa Asahara
Oct 8, 1999·Molecular Biology of the Cell·M W KlymkowskyY E Vourgourakis

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Adenomatous Polyposis Coli

Adenomatous polyposis coli is a protein encoded by the APC gene and acts as a tumor suppressor. Discover the latest research on adenomatous polyposis coli here.