Axis of serotonin -pERK-YAP in liver regeneration

Life Sciences
Yu FangSushun Liu

Abstract

Serotonin and YAP exhibit a vital role in regulating cell proliferation and wound-healing response. The aim of the study was to investigate whether 5-HT could promote liver regeneration by activating YAP. PH models were established by WT and TPH1-/- mice. ELISA, RT-PCR, western blot, immunohistochemistry, flow cytometry and MTT assay were used to assess the level of 5-HT and YAP and proliferation after PH. We found that 5-HT level was lower in the serum and liver of TPH1-/- mice. After PH, TPH1-/- mice, lacking in 5-HT, demonstrated worse regenerative ability and suffered more severe liver injury. Additionally, YAP expression was also lower in TPH1-/- mice. Moreover, we found that YAP expression was prominent within the first three days following PH. Similarly, 5-HT could promote cell proliferation by upregulating YAP expression in L-O2 cells. As predicted, using YAP-siRNA sharply reduced the proliferative capacity mediated by 5-HT. Further study also indicated that ERK participated in the regulation of YAP induced by 5-HT. By using an ERK inhibitor, the YAP expression and cell proliferation induced by 5-HT were both suppressed. Although YAP-siRNA was used to block YAP expression, pERK and ERK expression were not affected. Take...Continue Reading

Citations

Oct 18, 2019·Experimental & Molecular Medicine·Chao HeSushun Liu
Feb 15, 2021·Cellular and Molecular Life Sciences : CMLS·Lucy LeBlancJonghwan Kim
Nov 14, 2020·International Journal of Molecular Sciences·Shintaro YagiShinji Uemoto
Aug 8, 2021·International Journal of Molecular Sciences·Ning LiuXiaoyu Wang
Dec 16, 2021·Endocrinology and Metabolism·Jiwon ParkChang-Myung Oh

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