AXL is a candidate receptor for SARS-CoV-2 that promotes infection of pulmonary and bronchial epithelial cells.

Cell Research
Shuai WangXu Li

Abstract

The current coronavirus disease 2019 (COVID-19) pandemic presents a global public health challenge. The viral pathogen responsible, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), binds to the host receptor ACE2 through its spike (S) glycoprotein, which mediates membrane fusion and viral entry. Although the role of ACE2 as a receptor for SARS-CoV-2 is clear, studies have shown that ACE2 expression is extremely low in various human tissues, especially in the respiratory tract. Thus, other host receptors and/or co-receptors that promote the entry of SARS-CoV-2 into cells of the respiratory system may exist. In this study, we found that the tyrosine-protein kinase receptor UFO (AXL) specifically interacts with the N-terminal domain of SARS-CoV-2 S. Using both a SARS-CoV-2 virus pseudotype and authentic SARS-CoV-2, we found that overexpression of AXL in HEK293T cells promotes SARS-CoV-2 entry as efficiently as overexpression of ACE2, while knocking out AXL significantly reduces SARS-CoV-2 infection in H1299 pulmonary cells and in human primary lung epithelial cells. Soluble human recombinant AXL blocks SARS-CoV-2 infection in cells expressing high levels of AXL. The AXL expression level is well correlated with SARS-Co...Continue Reading

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Citations

Mar 4, 2021·International Journal of Molecular Sciences·Marta TrevisanLuisa Barzon
Mar 6, 2021·Emerging Microbes & Infections·Kelvin Kai-Wang ToKwok-Yung Yuen
Mar 6, 2021·International Journal of Biological Macromolecules·Pedro F N SouzaRaquel C Montenegro
Apr 6, 2021·Journal of Pharmaceutical Analysis·Congshan JiangShemin Lu
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May 11, 2021·Journal of Medicinal Chemistry·Javier García-CárcelesAna Martínez
May 10, 2021·The Lancet. Respiratory Medicine·Marcin F OsuchowskiIgnacio Rubio
Jun 4, 2021·Journal of Medicinal Chemistry·Thanigaimalai Pillaiyar, Stefan Laufer
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May 28, 2021·Risk Management and Healthcare Policy·Xiang YuYupeng Dong
Jun 1, 2021·The Journal of Biological Chemistry·John P Evans, Shan-Lu Liu
May 18, 2021·Andrology·Edson BorgesDaniela Paes de Almeida Ferreira Braga
May 17, 2021·Pharmacology & Therapeutics·Juliana P VagoFons A J van de Loo
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Jul 1, 2021·Trends in Biochemical Sciences·Ruchao PengGeorge Fu Gao
Jul 3, 2021·International Journal of Molecular Sciences·Valeria De PasqualeLuigi Michele Pavone

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Datasets Mentioned

BETA
MDA-MB-231
MT121215

Methods Mentioned

BETA
single-cell sequencing
tandem affinity purification
transfection
pulled
pull-down
confocal microscopy
co-IP
bronchoalveolar
lavage
electrophoresis

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