Axonal degeneration, regeneration and ganglion cell death in a rodent model of anterior ischemic optic neuropathy (rAION).

Experimental Eye Research
Cheng ZhangSteven L Bernstein

Abstract

Using laser-induced photoactivation of intravenously administered rose Bengal in rats, we generated an ischemic infarction of the intrascleral portion of the optic nerve (ON) comparable to that which occurs in humans to investigate optic nerve axon degenerative events following optic nerve infarct and the potential for axon re-growth. Animals were euthanized at different times post infarct. Axon degeneration was evaluated with SMI312 immunolabeling, and GAP-43 immunostaining was used to identify axon regeneration. Terminal dUTP nick end labeling (TUNEL) was used to evaluate retinal ganglion cell (RGC) death. There was significant axon structural disruptinot ion at the anterior intrascleral portion of the ON by 3d post-infarct, extending to the posterior ON by 7d post-stroke. Destruction of normal axon structure and massive loss of axon fibers occurred by 2 weeks. GAP-43 immunoreactivity occurred in the anterior ON by 7d post-infarct, lasting 3-4 weeks, without extension past the primary ischemic lesion. TUNEL-positive cells in the RGC layer appeared by 7d post-insult. These results indicate that following induction of ischemic optic neuropathy, significant axon damage occurs by 3d post-infarct, with later neuronal death. Post-s...Continue Reading

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Citations

Jan 28, 2014·Biochemistry. Biokhimii︠a︡·O S GancharovaP P Philippov
Jul 28, 2013·Investigative Ophthalmology & Visual Science·Joyce K HoYaping Joyce Liao
Aug 12, 2014·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Begoña Arana LarreaNerea Martinez Alday
Dec 4, 2015·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Sang Woo ParkHwan Heo
Jul 17, 2016·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Elisabet De DompabloG Rebolleda
Jul 24, 2018·Brain Injury : [BI]·Yingli WangZhiqiang Shen
Jan 3, 2018·Journal of Magnetic Resonance Imaging : JMRI·Arnaud AttyéAlexandre Krainik
Jun 6, 2020·Investigative Ophthalmology & Visual Science·Kathrin KaehlerAlexandra Koschak
Nov 24, 2017·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Sonia Labrador VelandiaIvan Fernandez-Bueno
Nov 24, 2020·European Journal of Ophthalmology·Lu LiChangzheng Chen

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