Axonal Extensions along Corticospinal Tracts from Transplanted Human Cerebral Organoids.

Stem Cell Reports
Takahiro KitaharaJun Takahashi

Abstract

The reconstruction of lost neural circuits by cell replacement is a possible treatment for neurological deficits after cerebral cortex injury. Cerebral organoids can be a novel source for cell transplantation, but because the cellular composition of the organoids changes along the time course of the development, it remains unclear which developmental stage of the organoids is most suitable for reconstructing the corticospinal tract. Here, we transplanted human embryonic stem cell-derived cerebral organoids at 6 or 10 weeks after differentiation (6w- or 10w-organoids) into mouse cerebral cortices. 6w-organoids extended more axons along the corticospinal tract but caused graft overgrowth with a higher percentage of proliferative cells. Axonal extensions from 10w-organoids were smaller in number but were enhanced when the organoids were grafted 1 week after brain injury. Finally, 10w-organoids extended axons in cynomolgus monkey brains. These results contribute to the development of a cell-replacement therapy for brain injury and stroke.

Citations

Jan 16, 2021·Tissue Engineering. Part C, Methods·Renata Vieira de SáR Jeroen Pasterkamp
Mar 27, 2021·AJOB Neuroscience·Tsutomu SawaiHideya Sakaguchi
Jul 9, 2021·Frontiers in Cellular Neuroscience·Guini SongYue He
Aug 11, 2021·Translational Neurodegeneration·Feng-Chen BiYao Yao
Aug 28, 2021·International Journal of Molecular Sciences·Kaneyasu Nishimura, Kazuyuki Takata

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