Axonal outgrowth and neuronal apoptosis in cultured adult mouse dorsal root ganglion preparations: effects of neurotrophins, of inhibition of neurotrophin actions and of prior axotomy

Neuroscience
A EdströmD Tonge

Abstract

Dorsal root ganglia (L4 and L5) with attached spinal roots and nerve stumps were isolated from young adult mice and cultured in a layer of extracellular matrix material (matrigel). Within one day, a large number of axons grew out from the cut ends of the nerve and the dorsal root. The average outgrowth length was more than doubled by nerve growth factor, which also strongly increased the number of fibres, showing extensive branching. There was also a significant outgrowth stimulation by neurotrophin-3, but no observable effect by brain-derived neurotrophic factor. In preparations isolated and cultured six days after peripheral nerve transection in vivo, there was an increase in both the outgrowth length (about 1.5- to 2-fold) and in the number of axons. Stimulation of axonal outgrowth, which concerned outgrowth from both the peripheral nerve and the dorsal root, could be further enhanced by the addition of nerve growth factor to the culture. K-252a, a selective inhibitor of neurotrophin receptor-associated tyrosine kinase activity, did not affect either the normal outgrowth or the increased outgrowth in pre-axotomized preparations, at a concentration which abolished the stimulating effects by exogenous nerve growth factor and n...Continue Reading

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Citations

Mar 23, 2004·Molecular and Cellular Neurosciences·Tam Thanh QuachPappachan E Kolattukudy
Sep 21, 2000·Neuroscience·D A Tonge, P G Leclere
Apr 15, 2003·Molecular and Cellular Neurosciences·Per A R EkströmDavid A Tonge
Mar 16, 2000·International Journal of Experimental Pathology·V MatthewsP McMinn
Nov 17, 1999·The European Journal of Neuroscience·I GavazziJ Cohen
May 5, 1999·Journal of Anatomy·G Terenghi
Oct 9, 2007·Neuroreport·Hilary H SachsRichard E Zigmond
Jul 1, 1997·The European Journal of Neuroscience·G D SterneG Terenghi
Feb 25, 2009·The Canadian Journal of Neurological Sciences. Le Journal Canadien Des Sciences Neurologiques·Budd A Tucker, Karen M Mearow
Oct 11, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jon P NiemiRichard E Zigmond
Jan 26, 2005·The Journal of Comparative Neurology·Lu-Ting KuoMichael J Groves
Jul 19, 2005·Experimental Neurology·S E ShoemakerR E Zigmond
Sep 18, 2012·Experimental Neurology·Richard E Zigmond
Sep 23, 2014·Neuroscience·A DeFrancesco-LisowitzR E Zigmond
Mar 24, 2015·The Spine Journal : Official Journal of the North American Spine Society·Hyunchul KimAdam H Hsieh
Feb 28, 2002·Molecular and Cellular Neurosciences·Yih-Jing LeePeter A McNaughton
Jun 13, 1998·Journal of Neurotrauma·P A DijkhuizenJ Verhaagen

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis