PMID: 6401798Feb 1, 1983Paper

Axonal polypeptides cross-reactive with antibodies to neurofilament proteins

Journal of Neurochemistry
B A BrownC A Marotta

Abstract

Antibodies were prepared to mammalian CNS neurofilament proteins (NFPs) and the antibody specificities were compared using a sensitive immunoblotting method. This procedure was used to detect and characterize cross-reactive proteins and their degradation products in neurofilament preparations. NFPs were prepared by axon flotation. Rabbits were immunized with 200,000, 140,000, and 70,000 NFPs (200K, 140K, and 70K) that had been electrophoretically purified by polyacrylamide gel electrophoresis (PAGE). By immunohistofluorescence it was shown that all antisera stained similar filamentous structures in rat cerebellar neurons. By use of a horseradish peroxidase-conjugated indirect antibody procedure, however, differences were detected in the cross-reactivities of the antisera to rat NFPs, separated by PAGE and electrophoretically transferred to nitrocellulose membranes. Each antiserum exhibited strong binding to the homologous NFP and, thus, was suitable for the detection of cross-reactive polypeptides and proteolytic degradation products derived exclusively from the individual NFPs. Anti-200K, anti-140K, or anti-70K was applied to overloaded two-dimensional nitrocellulose blots of NFPs prepared by axon flotation. Each of the three ...Continue Reading

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Citations

Aug 1, 1992·Journal of the Neurological Sciences·R S BlackJ P Blass
Feb 1, 1992·Journal of the Neurological Sciences·J P BlassA C Baker
Jul 1, 1994·Journal of the Neurological Sciences·L A DeGiorgioJ P Blass
Mar 1, 1988·Journal of Neuroscience Methods·E M Sajdel-SulkowskaC A Marotta
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·U C DrägerJ Kleinschmidt
Jan 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·B Tate-OstroffC A Marotta
Apr 1, 1989·Journal of Neurosurgery·D J GowerM Tytell
Jun 1, 1988·Journal of the American Geriatrics Society·E M Sajdel-SulkowskaC A Marotta
Mar 1, 1986·Journal of Neurochemistry·M VitadelloS Schiaffino
Jan 1, 1988·Journal of Geriatric Psychiatry and Neurology·F M BenesC A Marotta
Aug 1, 1984·Journal of Neurochemistry·R A Nixon, C A Marotta
Mar 1, 1984·The Journal of Comparative Neurology·A SeigerH Björklund
Jan 1, 1986·Journal of Neuroscience Research·S K Pixley, C W Cotman

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