PMID: 9421453Jan 8, 1998Paper

Axonal structure and function after axolemmal leakage in the squid giant axon

Journal of Neurotrauma
P E Gallant, J A Galbraith

Abstract

Membrane leakage is a common consequence of traumatic nerve injury. In order to measure the early secondary effects of different levels of membrane leakage on axonal structure and function we studied the squid giant axon after electroporation at field strengths of 0.5, 1.0, 1.6, or 3.3 kV/cm. Immediately after mild electroporation at 0.5 kV/cm, 40% of the axons had no action potentials, but by 1 h all of the mildly electroporated axons had recovered their action potentials. Many large organelles (mitochondria) were swollen, however, and their transport was reduced by 62% 1 h after this mild electroporation. One hour after moderate electroporation at 1.0 kV/cm, most of the axons had no action potentials, most large organelles were swollen, and their transport was reduced by 98%, whereas small organelle transport was reduced by 75%. Finally at severe electroporation levels of 1.65-3.0 kV/cm all conduction and transport was lost and the gel-like axoplasmic structure was clumped or liquefied. The structural damage and transport block seen after severe and moderate poration were early secondary injuries that could be prevented by placing the porated axons in an intracellular-type medium (low in Ca2+, Na+, and Cl-) immediately after ...Continue Reading

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Citations

Jul 27, 2011·Journal of Neurotrauma·George C MagouBryan J Pfister
Apr 10, 1999·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·D O Okonkwo, J T Povlishock
Jul 27, 2012·Medical Physics·Radia Abdul WahabMatthew R Myers
Sep 2, 1998·Proceedings of the National Academy of Sciences of the United States of America·J A LundqvistO Orwar
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·J A GalbraithP E Gallant
Jan 1, 2010·Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia·Hong-Cai Wang, Yan-Bin Ma
Feb 9, 2005·Journal of Neuroscience Methods·Helen A McNallyJ Paul Robinson
Sep 22, 2005·Journal of Neuroscience Methods·Peter LovellLeonid L Moroz
Jun 17, 2018·International Journal for Numerical Methods in Biomedical Engineering·Ilaria CinelliMaeve Duffy
May 22, 2009·Journal of Neural Engineering·Ethan D Cohen
Nov 22, 2017·International Journal for Numerical Methods in Biomedical Engineering·I CinelliP McHugh
Oct 16, 2020·Progress in Biophysics and Molecular Biology·Christian FillaferMatthias F Schneider

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