Axonal transport: a quantitative study of retained and transported protein fraction in the cat

Journal of Neurobiology
E J Muñoz-MartínezA Sanderson

Abstract

The fast axonal transport of proteins was studied in the cat sciatic nerve after injection of [3H]leucine into the spinal ganglion or the ventral horn of the seventh lumbar segment. The amount of transported proteins after ganglion injection was linearly related to the amount of label present at the ganglion. At variable intervals after ganglion or spinal cord injection, the sciatic nerves were sectioned in some experiments. The transport of proteins continued in the peripheral nerve stump in a wavelike manner, but the advancing wave leaves a labeled trail behind. A fraction of this trail corresponds to proteins moving at slower velocities than the velocity of proteins in the wave front. Another fraction of the trail corresponds to molecules retained by the axons. Each nerve segment of 5 mm in length retains 1.5% of the transported proteins, and the profile of retained proteins along the sciatic nerves follows a single exponential function. From the proportion of retained proteins, the concentration of transported proteins at the terminals of branching axons as a function of the branching ratio was estimated. In the case of motor axons innervating the soleus muscle of the cat, the concentration of recently transported proteins ...Continue Reading

References

Feb 1, 1976·The Journal of Physiology·S Ochs
Aug 1, 1977·Journal of Neurochemistry·M A Bisby, V T Bulger
Aug 1, 1979·Experimental Neurology·E J Muńoz-Martinez, B Chavez
Jun 1, 1971·Journal of Neurochemistry·J O Karlsson, J Sjöstrand
May 1, 1971·Journal of Neurochemistry·J O Karlsson, J Sjöstrand
Dec 1, 1972·The Journal of Physiology·S Ochs
Jul 1, 1969·Journal of Neurochemistry·A M Kidwai, S Ochs
Sep 1, 1968·The Journal of Cell Biology·B S McEwen, B Grafstein
May 1, 1968·Experimental Neurology·R J Lasek
Jan 1, 1965·Journal of Neurophysiology·A M MCPHEDRANE HENNEMAN

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Citations

Jan 1, 1989·Neurochemistry International·S Ochs
Sep 1, 1984·Journal of Neurobiology·E J Muñoz-MartínezS Ochs
Jul 1, 1982·Experimental Neurology·D P Stromska, S Ochs
Nov 1, 1983·Experimental Neurology·A Hernández-Cruz, E J Muñoz-Martínez
Feb 1, 1985·Journal of Neurochemistry·C D Carrington, M B Abou-Donia
Jan 1, 1984·Neuropathology and Applied Neurobiology·A Hernández-Cruz, E J Muñoz-Martínez
Mar 1, 1983·Neuropathology and Applied Neurobiology·E J Muñoz-MartínezP Joseph-Nathan
Aug 15, 1996·Microscopy Research and Technique·K P Gatzinsky
Sep 1, 1981·Journal of Neurobiology·D P Stromska, S Ochs
Sep 1, 1982·Journal of Neurobiology·D W O'Brien, R E Snyder
Mar 1, 1983·Journal of Neurobiology·G SchmidD G Weiss

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