PMID: 6166619Jun 1, 1981Paper

Axonal transport of calmodulin: a physiologic approach to identification of long-term associations between proteins

The Journal of Cell Biology
S T BradyR J Lasek

Abstract

Calmodulin is a soluble, heat-stable protein which has been shown to modulate both membrane-bound and soluble enzymes, but relatively little has been known about the in vivo associations of calmodulin. A 17,000-dalton heat-stable protein was found to move in axonal transport in the guinea pig visual system with the proteins of slow component b (SCb; 2 mm/d) along with actin and the bulk of the soluble proteins of the axon. Co-electrophoresis of purified calmodulin and radioactively labeled SCb proteins in two dimensional polyacrylamide gel electrophoresis (PAGE) demonstrated the identity of the heat-stable SCb protein and calmodulin on the basis of pI, molecular weight, and anomalous migration in the presence of Ca2+-chelating agents. No proteins co-migrating with calmodulin in two-dimensional PAGE could be detected among the proteins of slow component a (SCa; 0.3 mm/d, microtubules and neurofilaments) or fast component (FC; 250 mm/d, membrane-associated proteins). We conclude that calmodulin is transported solely as part of the SCb complex of proteins, the axoplasmic matrix. Calmodulin moves in axonal transport independent of the movements of microtubules (SCa) and membranes (FC), which suggests that the interactions of calmod...Continue Reading

References

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Citations

Jan 1, 1992·Molecular Neurobiology·J L Cyr, S T Brady
Mar 24, 1987·Brain Research·D J Gower, M Tytell
Jan 1, 1986·Journal of the Neurological Sciences·D J Gower, M Tytell
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May 1, 1992·The Journal of Cell Biology·R J LasekM J Katz
Jul 1, 1984·The Journal of Cell Biology·R J LasekS T Brady
Nov 1, 1984·The Journal of Cell Biology·S T BradyR J Lasek
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May 16, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Subhojit RoyVirginia M-Y Lee
Sep 27, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Yong TangSubhojit Roy
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Nov 4, 1982·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·R J Lasek
Apr 1, 1985·Journal of Dental Research·J E Aubin

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