Axotomy-induced apoptotic cell death of neonatal rat facial motoneurons: time course analysis and relation to NADPH-diaphorase activity

Experimental Neurology
J P RossiterM A Bisby

Abstract

Rapid and massive death of motoneurons occurs following axotomy in neonatal mammals. This likely results from the neurons being deprived of access to target-derived trophic factors, as their death can be prevented by application of a variety of neurotrophic factors to the proximal end of the cut nerve. Since trophic factor-deprived embryonic chick motoneurons undergo apoptosis in vitro, we have investigated whether axotomized neonatal rat facial motoneurons undergo apoptotic cell death in vivo. Following facial nerve transection during the first postnatal day, the dying motoneurons show characteristic morphological changes of apoptosis and undergo DNA fragmentation, as detected by an in situ end labeling technique. An initial sharp burst of DNA fragmentation, between 12 and 24 h postaxotomy, accompanies a steep decline in neuronal numbers, indicating that neuronal cell death rapidly follows endonuclease cleavage of DNA. However, the interval between axotomy and onset of DNA fragmentation varies widely. By 4 days postnatum only 38% of the lesioned motoneurons have survived an initial rapid phase of neuronal loss, whereas 11% survive to 10 days postnatum at least. NADPH-diaphorase/nitric oxide synthase activity has been implicate...Continue Reading

Citations

Dec 31, 2005·Neuropathology : Official Journal of the Japanese Society of Neuropathology·Kazuhiko WatabeYoko Kawazoe
Sep 9, 2004·The Journal of Cell Biology·Jacqueline L VanderluitRuth S Slack
Jul 11, 2000·The European Journal of Neuroscience·D PerreletA C Kato
Jul 12, 2002·Journal of Neuroscience Research·Laia AcarinBernardo Castellano
Oct 22, 2002·The Journal of Comparative Neurology·Tiziana BorselloPeter G H Clarke
Mar 26, 2009·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Yoshihiro SekiToru Iwaki
May 26, 2010·Neuropathology : Official Journal of the Japanese Society of Neuropathology·Yukio FujitaKoichi Okamoto
Jul 21, 2006·Forensic Science International : Synergy·Luyang TaoShizhong Bian
Dec 20, 2005·Neuroscience Letters·Amauri Pierucci, Alexandre Leite Rodrigues de Oliveira
Jan 26, 2000·Journal of Neurochemistry·R S ClarkS H Graham
Apr 27, 2017·Plastic and Reconstructive Surgery·Joseph CatapanoGregory H Borschel
Jul 3, 1998·The Journal of Comparative Neurology·R Wetts, J E Vaughn
Jan 28, 2010·Pharmaceuticals·Thierry BordetRebecca M Pruss
May 20, 1998·Neuroreport·M ShibayamaA Tessler
May 1, 2002·Clinical Neuropharmacology·Merziah Panahi, Taki Al-Tiraihi
Oct 23, 2002·Journal of Neuroscience Research·Bruce Teter, J Wesson Ashford
Feb 16, 1999·Journal of Neurotrauma·W H HuM F Jen
Feb 1, 1997·Molecular Neurobiology·S Y Fu, T Gordon
May 5, 2001·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·T Arendt
May 4, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·R S ClarkS H Graham

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis