PMID: 8968729Nov 1, 1996Paper

Axotomy-induced loss of m2 muscarinic receptor mRNA in the rat facial motor nucleus precedes a decrease in concentration of muscarinic receptors

The Histochemical Journal
D B HooverJ V Lewis

Abstract

The abundance of muscarinic receptors and m2 muscarinic receptor mRNA in the facial nuclei of rats was evaluated by autoradiographic procedures at various times up to 14 days after transection of the right facial nerve. Receptors were labelled by in vitro incubation of brain sections with L-[3H]quinuclidinyl benzilate, while in situ hybridization with a 35S-labelled oligonucleotide was used to identify m2 muscarinic receptor mRNA in neighbouring sections. The right and left facial nuclei of non-operated control rats appeared equivalent in abundance of muscarinic receptors (359 +/- 8 versus 376 +/- 9 fmol per mg tissue, n = 5) and the presence of m2 mRNA. Axotomy had no effect on the concentration of receptors in the contralateral facial nucleus but caused a gradual loss of receptors from the ipsilateral side. No change was detected at 1 day after nerve transection, but a 23% decrease relative to the contralateral facial nucleus had occurred by 3 days. A maximum decrease of 51% was achieved by 1 week after nerve transection. By comparison, m2 mRNA was nearly eliminated from the ipsilateral facial nucleus at 1 day post-taxonomy and remained depleted for the duration of study. Previous work has established that no significant loss...Continue Reading

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Citations

Mar 9, 2004·Brain Research. Brain Research Reviews·Linda B Moran, Manuel B Graeber
Mar 6, 2013·Brain Research·Toshihumi IchimiyaKazuyuki Nakajima
Apr 29, 2005·Brain Research. Molecular Brain Research·Isabelle VassiasCatherine de Waele
Sep 24, 1998·Trends in Pharmacological Sciences· Haddad el-B, J Rousell
Nov 25, 2005·Journal of Neuroscience Research·Yong-Ming Jin, Donald A Godfrey

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