Azelastine hydrochloride inhibits platelet activating factor-like activity in human eosinophils

Prostaglandins, Leukotrienes, and Essential Fatty Acids
K Shindo, M Fukumura

Abstract

We investigated the inhibitory effect of azelastine hydrochloride (azelastine), an anti-asthmatic drug, on platelet-activating factor (PAF)-like activity in eosinophils obtained from asthmatic and non-asthmatic patients. Eosinophils were preincubated with or without azelastine and stimulated with f-Met-Leu-Phe (fMLP, 10 mumol) for 15 min. PAF-like activity was detected by aggregation of washed guinea-pig platelets. PAF-like activity released from asthmatic eosinophils without preincubation of azelastine was 2.36 [1.02] (mean [SD], ng/10(7) cells) in supernatants and 13.87 [4.77] in cell pellets. After preincubation with 10(-8), 10(-6), and 10(-4) M azelastine, PAF-like activity reduced to 1.85 [0.46] (mean [SD], ng/10(7) cells), 1.11 [0.14], and 0.88 [0.09] (n = 15) in the supernatants, and 11.83 [2.93], 8.32 [1.41], and 6.27 [1.25] (n = 15) in the cell pellets, respectively. PAF-like activity in non-asthmatic eosinophils without preincubation of azelastine was 2.01 [0.86] (mean [SD], ng/10(7) cells) in supernatants and 7.44 [0.99] in cell pellets. After preincubation with 10(-8), 10(-6), and 10(-4) M azelastine, PAF-like activity reduced to 1.73 [0.64] (mean [SD], ng/10(7) cells), 1.12 [0.23], and 0.84 [0.17] (n = 20) in the s...Continue Reading

References

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Citations

Mar 29, 2001·Drugs·G M WalshH Timmerman
May 1, 1997·Prostaglandins, Leukotrienes, and Essential Fatty Acids·K ShindoK Koide
May 13, 1998·Prostaglandins, Leukotrienes, and Essential Fatty Acids·K Shindo, M Fukumura
Feb 7, 1998·Prostaglandins, Leukotrienes, and Essential Fatty Acids·K ShindoM Fukumura

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