Azithromycin and Chloramphenicol Diminish Neutrophil Extracellular Traps (NETs) Release

International Journal of Molecular Sciences
W BystrzyckaOlga Ciepiela

Abstract

Neutrophils are one of the first cells to arrive at the site of infection, where they apply several strategies to kill pathogens: degranulation, respiratory burst, phagocytosis, and release of neutrophil extracellular traps (NETs). Antibiotics have an immunomodulating effect, and they can influence the properties of numerous immune cells, including neutrophils. The aim of this study was to investigate the effects of azithromycin and chloramphenicol on degranulation, apoptosis, respiratory burst, and the release of NETs by neutrophils. Neutrophils were isolated from healthy donors by density-gradient centrifugation method and incubated for 1 h with the studied antibiotics at different concentrations (0.5, 10 and 50 μg/mL-azithromycin and 10 and 50 μg/mL-chloramphenicol). Next, NET release was induced by a 3 h incubation with 100 nM phorbol 12-myristate 13-acetate (PMA). Amount of extracellular DNA was quantified by fluorometry, and NETs were visualized by immunofluorescent microscopy. Degranulation, apoptosis and respiratory burst were assessed by flow cytometry. We found that pretreatment of neutrophils with azithromycin and chloramphenicol decreases the release of NETs. Moreover, azithromycin showed a concentration-dependent e...Continue Reading

References

Mar 1, 1978·Antimicrobial Agents and Chemotherapy·M M SekleckiE G Maderazo
May 1, 1979·Antimicrobial Agents and Chemotherapy·J R KoupR L Slaughter
Apr 1, 1977·Antimicrobial Agents and Chemotherapy·A Forsgren, D Schmeling
May 1, 1982·The American Journal of Medicine·W E Hauser, J S Remington
Jul 1, 1996·Infection·B Van VlemS Ringoir
Aug 13, 1997·European Journal of Pharmacology·D HoebenC Burvenich
Nov 17, 2001·International Journal of Antimicrobial Agents·W L Hand, D L Hand
May 16, 2002·Pharmacotherapy·Daniel P HealyGeorge E Babcock
Nov 1, 1950·Proceedings of the Society for Experimental Biology and Medicine·J MUNOZ, R GEISTER
May 27, 2005·Antimicrobial Agents and Chemotherapy·Martina BosnarMichael J Parnham
Nov 23, 2011·European Journal of Clinical Pharmacology·P ZarogoulidisK Zarogoulidis
Dec 23, 2011·The Journal of Antimicrobial Chemotherapy·Alexandra KovalevaJan Jelrik Oosterheert
Jul 13, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Joanna Skrzeczynska-MoncznikJoanna Cichy
Jan 30, 2013·Antimicrobial Agents and Chemotherapy·P MatznellerM Zeitlinger
Jun 5, 2013·Seminars in Immunopathology·Nora Branzk, Venizelos Papayannopoulos
Sep 7, 2013·Blood·Bryan G Yipp, Paul Kubes
Mar 20, 2014·Journal of Innate Immunity·Natalja JerjomicevaMaren von Köckritz-Blickwede
Jan 1, 2014·Central-European Journal of Immunology·Aneta MandaUrszula A Demkow
Dec 9, 2015·Antimicrobial Agents and Chemotherapy·Theocharis KonstantinidisKonstantinos Ritis
Apr 5, 2016·Journal of Clinical and Diagnostic Research : JCDR·Smita Sood
Apr 21, 2016·Central-European Journal of Immunology·Weronika BystrzyckaOlga Ciepiela
Nov 9, 2016·Advances in Experimental Medicine and Biology·A Manda-HandzlikO Ciepiela

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Citations

Apr 12, 2018·International Journal of Molecular Sciences·Ronald AndersonCharles Feldman
Jun 12, 2019·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Jae Woong HwangSang Hag Lee
Dec 8, 2019·American Journal of Respiratory and Critical Care Medicine·Lisa Saiman, Michael S Schechter
May 20, 2020·Infection and Immunity·Kerstin PohlKenneth C Malcolm
Aug 15, 2020·Frontiers in Cellular and Infection Microbiology·Alexander J CurrieAdilia Warris
Sep 3, 2020·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Binbin ZhuXiuming Jin
Aug 3, 2020·Clinical Reviews in Allergy & Immunology·Victoria Mutua, Laurel J Gershwin
Nov 10, 2020·Frontiers in Pharmacology·Chih-Chao ChiangTsong-Long Hwang
Mar 2, 2021·Frontiers in Immunology·Vincent J VendittoDavid J Feola
Mar 7, 2021·International Journal of Molecular Sciences·Alessandra GhigoVirginia De Rose
Mar 7, 2021·International Journal of Molecular Sciences·Isaac MartinHartmut Grasemann
May 1, 2021·Antibiotics·Rosa María Girón MorenoRosa Mar Gómez-Punter
May 11, 2021·The Journal of Allergy and Clinical Immunology·Luís AlmeidaTim Sparwasser
May 21, 2021·European Journal of Pharmacology·Mohammad Rafi KhezriReza Jafari
Jul 25, 2021·International Journal of Molecular Sciences·Nicole SteffensenNicole de Buhr

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Methods Mentioned

BETA
flow cytometry
PMA
density-gradient centrifugation

Software Mentioned

GraphPad
GraphPad Prism

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