Azithromycin inhibits interleukin-6 but not fibrinogen production in hepatocytes infected with cytomegalovirus and chlamydia pneumoniae

The Journal of Laboratory and Clinical Medicine
J J M BouwmanR J A Diepersloot

Abstract

Chlamydia pneumoniae and cytomegalovirus (CMV) have been associated with the development of atherosclerosis. Inflammatory stimuli initiate the biosynthesis of fibrinogen, interleukin (IL)-6 and plasminogen activator inhibitor (PAI)-1 in the liver. Chronic infection may perpetuate the inflammatory status. We hypothesized that infection of human hepatocytes with the intracellular pathogens C pneumoniae and CMV accelerates biosynthesis of fibrinogen, IL-6, and PAI-1 but that this biosynthesis can be reduced with the use of azithromycin. HepG2 human hepatocytes were infected with C pneumoniae and CMV in vitro in the presence of 0, 0.016, 0.125, or 1 microg/mL azithromycin. We measured IL-6, PAI-1, and fibrinogen after 24, 48, 72, and 96 hours. C pneumoniae-infected hepatocytes produce IL-6 (2667 +/- 309 pg/mL vs 137 +/- 120 pg/mL in uninfected cells after 96 hours. Incubation with 0.016 microg/mL azithromycin decreased IL-6 levels to a mean of 1516 +/- 402 pg/mL, and incubation with 0.125 and 1 microg/mL azithromycin decreased IL-6 to 871 +/- 364 and 752 +/- 403 pg/mL, respectively. C pneumoniae-induced IL-6 production was time- and dose-dependent. The interaction of C pneumoniae with azithromycin treatment was significant, indicat...Continue Reading

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Citations

Jul 1, 2005·Digestive Diseases and Sciences·Fusun F BolukbasIlyas Ozardali
Mar 19, 2008·International Journal of Obesity : Journal of the International Association for the Study of Obesity·J J M BouwmanR J A Diepersloot
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Apr 4, 2014·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Yureeda QaziPedram Hamrah
Sep 1, 2005·European Journal of Clinical Investigation·J J M BouwmanR J A Diepersloot
Feb 9, 2013·Annals of Plastic Surgery·Ping YangXuan-Fen Zhang
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Jun 8, 2021·Frontiers in Immunology·Wenjing XiangZhou Zhou

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