Azithromycin inhibits mucus hypersecretion from airway epithelial cells.

Mediators of Inflammation
T Shimizu, Shino Shimizu

Abstract

To examine the in vivo effects of the 15-member macrolide, azithromycin (AZM), on mucus hypersecretion, we induced hypertrophic and metaplastic changes of goblet cells in rat nasal epithelium by intranasal instillation of ovalbumin (OVA) in OVA-sensitized rats, or by intranasal lipopolysaccharides (LPS) instillation. Oral administration of AZM (5-10 mg/kg) or clarithromycin (CAM, 5-10 mg/kg) significantly inhibited OVA- and LPS-induced mucus production, whereas josamycin (JM) or ampicillin (ABPC) showed no effect. In vitro effects of AZM on airway epithelial cells were examined using NCI-H292 cells and human nasal epithelial cells cultured in air-liquid interface. Mucus secretion was evaluated by enzyme-linked immunosorbent assay using an anti-MUC5AC monoclonal antibody. AZM or CAM significantly inhibited tumor necrosis factor-α (TNF-α) (20 ng/mL)-induced MUC5AC secretion from NCI-H292 cells at 10⁻⁶-10⁻⁷ M, whereas JM or ABPC showed no effect. AZM significantly inhibited TNF-α (20 ng/mL)-induced MUC5AC secretion from human nasal epithelial cells at 10⁻⁴ M. MUC5AC mRNA expression was also significantly inhibited. These results indicate that the 15-member macrolide, AZM, exerts direct inhibitory effects on mucus secretion from ai...Continue Reading

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Citations

Jun 4, 2014·Drugs·Clémence MartinPierre-Régis Burgel
May 25, 2015·Revue des maladies respiratoires·M AbelyM Fayon
Aug 28, 2020·Expert Review of Anti-infective Therapy·Daniel Echeverría-EsnalSantiago Grau
Oct 14, 2017·Journal of Biophotonics·Aleksey LantsovAleksey Shchekin
Jun 13, 2021·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Kim ThomsenClaus Moser

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay

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