AzoCholine Enables Optical Control of Alpha 7 Nicotinic Acetylcholine Receptors in Neural Networks.

ACS Chemical Neuroscience
Arunas DamijonaitisDirk Trauner

Abstract

Nicotinic acetylcholine receptors (nAChRs) are essential for cellular communication in higher organisms. Even though a vast pharmacological toolset to study cholinergic systems has been developed, control of endogenous neuronal nAChRs with high spatiotemporal precision has been lacking. To address this issue, we have generated photoswitchable nAChR agonists and re-evaluated the known photochromic ligand, BisQ. Using electrophysiology, we found that one of our new compounds, AzoCholine, is an excellent photoswitchable agonist for neuronal α7 nAChRs, whereas BisQ was confirmed to be an agonist for the muscle-type nAChR. AzoCholine could be used to modulate cholinergic activity in a brain slice and in dorsal root ganglion neurons. In addition, we demonstrate light-dependent perturbation of behavior in the nematode, Caenorhabditis elegans.

References

Apr 1, 1987·Physiological Reviews·A M Gurney, H A Lester
May 1, 1974·Genetics·S Brenner
Jul 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·J BiethB F Erlanger
Dec 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·W J DealD Nachmansohn
Aug 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·E BartelsB F Erlanger
Aug 15, 2000·Behavioural Brain Research·C GottiF Clementi
Aug 7, 2004·Autonomic Neuroscience : Basic & Clinical·Rainer Viktor HaberbergerWolfgang Kummer
Nov 24, 2004·Nature Neuroscience·Matthew BanghartRichard H Kramer
Feb 11, 2005·Journal of Molecular Biology·Nigel Unwin
Mar 12, 2005·The Journal of Physiology·Sergio FucileFabrizio Eusebi
Dec 2, 2005·Proceedings of the National Academy of Sciences of the United States of America·Thomas GrutterJean-Pierre Changeux
Jul 27, 2007·The FEBS Journal·Dimitra KalamidaSocrates J Tzartos
Aug 9, 2008·PLoS Biology·Stacey L EdwardsKenneth G Miller
Oct 13, 2010·The Journal of Physiology·Christina NassensteinBradley J Undem
Nov 24, 2011·Angewandte Chemie·Timm FehrentzDirk Trauner
Feb 22, 2012·Nature Methods·Alexandre MourotRichard H Kramer
Apr 21, 2012·Angewandte Chemie·Philipp StawskiDirk Trauner
Sep 13, 2012·Angewandte Chemie·Marco SteinDirk Trauner
Sep 20, 2012·Chemical Reviews·Damien LemoineThomas Grutter
Mar 5, 2013·Nature Neuroscience·Joshua LevitzEhud Y Isacoff
Dec 4, 2013·Proceedings of the National Academy of Sciences of the United States of America·Damien LemoineThomas Grutter
Jan 25, 2014·Journal of the American Chemical Society·Willem A VelemaBen L Feringa
Feb 13, 2014·Angewandte Chemie·Matthias Schönberger, Dirk Trauner
May 27, 2014·ACS Chemical Neuroscience·Matthias SchoenbergerDirk Trauner
Jun 5, 2014·Angewandte Chemie·Johannes BroichhagenDirk Trauner

❮ Previous
Next ❯

Citations

May 11, 2016·ACS Central Science·Johannes BroichhagenDirk Trauner
Sep 20, 2015·Chemistry : a European Journal·Jaume Garcia-AmorósDolores Velasco
Jun 24, 2015·Accounts of Chemical Research·Johannes BroichhagenDirk Trauner
Apr 8, 2017·Science·Brenda C ShieldsMichael R Tadross
Dec 14, 2017·Biologie aujourd'hui·Romain Durand-de CuttoliAlexandre Mourot
Sep 1, 2017·British Journal of Pharmacology·Piotr BregestovskiPau Gorostiza
May 19, 2017·Angewandte Chemie·Nicholas AnkenbruckAlexander Deiters
Apr 12, 2017·EMBO Reports·Shai Berlin, Ehud Y Isacoff
Dec 7, 2019·Beilstein Journal of Organic Chemistry·Lorenzo SansaloneGraham C R Ellis-Davies
Jul 11, 2019·Nature Reviews. Neuroscience·Pierre PaolettiAlexandre Mourot
Jun 15, 2019·Proceedings of the National Academy of Sciences of the United States of America·Silvia PittoloPau Gorostiza
Dec 19, 2017·Physical Chemistry Chemical Physics : PCCP·Jaume Garcia-AmorósDolores Velasco
Jan 17, 2017·Chemical Science·David M BarberDirk Trauner
Feb 24, 2019·Nature Communications·Gisela CabréRamon Alibés
Aug 14, 2020·Chembiochem : a European Journal of Chemical Biology·Johannes MorsteinDirk Trauner
Jan 18, 2017·ACS Chemical Neuroscience·Craig W LindsleyAnne M Andrews
Apr 11, 2020·ACS Chemical Neuroscience·Bichu ChengDirk Trauner
May 2, 2019·ACS Central Science·Johannes MorsteinDirk Trauner
Jul 10, 2018·Chemical Reviews·Katharina HüllDirk Trauner

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.