Azole resistance in Candida glabrata: coordinate upregulation of multidrug transporters and evidence for a Pdr1-like transcription factor

Antimicrobial Agents and Chemotherapy
John-Paul Vermitsky, Thomas D Edlind

Abstract

Candida glabrata has emerged as a common cause of fungal infection. This yeast has intrinsically low susceptibility to azole antifungals such as fluconazole, and mutation to frank azole resistance during treatment has been documented. Potential resistance mechanisms include changes in expression or sequence of ERG11 encoding the azole target. Alternatively, resistance could result from upregulated expression of multidrug transporter genes; in C. glabrata these include CDR1 and PDH1. By RNA hybridization, 10 of 12 azole-resistant clinical isolates showed 6- to 15-fold upregulation of CDR1 compared to susceptible strains. In 4 of these 10 isolates PDH1 was similarly upregulated, and in the remainder it was upregulated three- to fivefold, while ERG11 expression was minimally changed. Laboratory mutants were selected on fluconazole-containing medium with glycerol as carbon source (to eliminate mitochondrial mutants). Similar to the clinical isolates, six of seven laboratory mutants showed unchanged ERG11 expression but coordinate CDR1-PDH1 upregulation ranging from 2- to 20-fold. Effects of antifungal treatment on gene expression in susceptible C. glabrata strains were also studied: azole exposure induced CDR1-PDH1 expression 4- to...Continue Reading

References

Apr 1, 1991·Journal of Bacteriology·S M BarnsW G Weisburg
Feb 1, 1995·Journal of Bioenergetics and Biomembranes·E Balzi, A Goffeau
Dec 1, 1996·Antimicrobial Agents and Chemotherapy·G D AlbertsonH F Jenkinson
Apr 28, 1997·Molecular & General Genetics : MGG·G Del SorboM A De Waard
Jul 14, 1999·Journal of Medical Microbiology·A DefontaineJ N Hallet
Feb 29, 2000·Journal of Bacteriology·L E CowenL M Kohn
May 19, 2000·Antimicrobial Agents and Chemotherapy·F BarchiesiG Scalise
Sep 19, 2000·Antimicrobial Agents and Chemotherapy·K W HenryT D Edlind
Aug 10, 2001·Microbiology·M WeigF A Mühlschlegel
Aug 11, 2001·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Anna Kolaczkowska, Andre Goffeau
Mar 29, 2002·Molecular Genetics and Genomics : MGG·A KolaczkowskaA Goffeau
Apr 12, 2002·The Journal of Biological Chemistry·Bassel Akache, Bernard Turcotte
May 17, 2002·The Journal of Hospital Infection·V Krcmery, A J Barnes
May 23, 2002·Antimicrobial Agents and Chemotherapy·Theodore C WhiteDavid A Stevens
Oct 2, 2002·Journal of Clinical Microbiology·A-M FreydièreF C Odds
Jan 31, 2003·Yeast·Koichi IzumikawaJohn E Bennett
Sep 25, 2003·Antimicrobial Agents and Chemotherapy·Luis Ostrosky-ZeichnerJeannette Lee
Apr 24, 2004·Antimicrobial Agents and Chemotherapy·John E BennettKieren A Marr

❮ Previous
Next ❯

Citations

Oct 27, 2006·Current Infectious Disease Reports·Katherine S Barker, P David Rogers
May 17, 2005·Diagnostic Microbiology and Infectious Disease·Martin BardKevin C Hazen
Jun 16, 2010·Antimicrobial Agents and Chemotherapy·Huei-Fung TsaiJohn E Bennett
Sep 9, 2010·Antimicrobial Agents and Chemotherapy·Thomas D Edlind, Santosh K Katiyar
May 1, 2013·Antimicrobial Agents and Chemotherapy·Catarina CostaMiguel C Teixeira
Nov 19, 2008·Antimicrobial Agents and Chemotherapy·Erwin LampingRichard D Cannon
Apr 27, 2011·Antimicrobial Agents and Chemotherapy·A P SilvaA G Rodrigues
Dec 12, 2012·Antimicrobial Agents and Chemotherapy·Jason A NobleJohn E Bennett
May 28, 2009·Antimicrobial Agents and Chemotherapy·Monika SharmaRajendra Prasad
Oct 28, 2005·Antimicrobial Agents and Chemotherapy·Patrick VandeputteJean-Philippe Bouchara
Apr 16, 2009·Clinical Microbiology Reviews·Richard D CannonBrian C Monk
Jan 5, 2011·Eukaryotic Cell·Kelly E CaudleP David Rogers
Sep 18, 2007·Eukaryotic Cell·Kailash Gulshan, W Scott Moye-Rowley
Dec 7, 2010·Eukaryotic Cell·Sanjoy PaulW Scott Moye-Rowley
Sep 8, 2005·Journal of Clinical Microbiology·Brent T Page, Cletus P Kurtzman
Sep 29, 2011·Canadian Journal of Microbiology·Nora Toth HervayYvetta Gbelska
Dec 22, 2011·International Journal of Microbiology·Patrick VandeputteAlix T Coste
Nov 30, 2011·Future Microbiology·Zeljkica Jandric, Christoph Schüller
Feb 6, 2014·World Journal of Microbiology & Biotechnology·Pendru RaghunathK Subbannayya
Nov 20, 2013·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·C F RodriguesM Henriques
Jul 24, 2013·BioMed Research International·Claudia Spampinato, Darío Leonardi
Feb 24, 2006·Antimicrobial Agents and Chemotherapy·Eva J HelmerhorstFrank G Oppenheim
Sep 11, 2009·Current Opinion in Infectious Diseases·David S Perlin
May 27, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Cristiane S Shinobu-MesquitaTerezinha I E Svidzinski
Jan 24, 2016·The Journal of Antimicrobial Chemotherapy·Anna Kołaczkowska, Marcin Kołaczkowski
Oct 4, 2011·Veterinary Microbiology·Claudia CafarchiaDomenico Otranto
Nov 16, 2010·International Journal of Antimicrobial Agents·N KondoriI Mattsby-Baltzer
Jul 22, 2009·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Vincenzo SaviniDomenico D'Antonio
Feb 7, 2009·International Journal of Antimicrobial Agents·Norbert BerilaJulius Subik

❮ Previous
Next ❯

Related Concepts

Related Feeds

Candidiasis (ASM)

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

CRISPR Screens in Drug Resistance

CRISPR-Cas system enables the editing of genes to create or correct mutations. This feed focuses on the application of CRISPR-Cas system in high-throughput genome-wide screens to identify genes that may confer drug resistance.

Candidiasis

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.