PMID: 6025122Jun 23, 1967Paper

Azure mutants: a type of host-dependent mutant of the bacteriophage f2

Science
K Horiuchi, N D Zinder

Abstract

A new type of host-dependent mutant, azure mutant, of bacteriophage f2 has been isolated. Growth of these mutants was restricted specifically by amber suppressor genes in the host bacteria. Restriction of the formation of infective centers by different bacterial suppressor genes was 98 percent, 90 percent, and 70 percent with Su-3, Su-1, and Su-2 genes, respectively. Restriction, like suppression, was the dominant phenotype. The block in growth of the mutants occurred in an early stage of the infection cycle. Once infection was established, however, an infected cell produced approximately the same number of progeny phage as a cell without the suppressor genes did. It is proposed that the azure codon is the same as the amber codon (uracil, adenine, guanine) and that restriction results from improper termination of protein chains of the phage RNA polymerase. Similar mutants may exist in other systems.

References

Dec 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·D L EngelhardtN Zinder
Jan 1, 1965·Annual Review of Microbiology·N D Zinder
Dec 1, 1965·Journal of Molecular Biology·S KaplanS Brenner
May 1, 1961·Virology·A CAMPBELL
Jul 15, 1962·Proceedings of the National Academy of Sciences of the United States of America·S BENZER, S P CHAMPE
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Jan 4, 1964·Nature·A S SARABHAIA BOLLE
Jun 1, 1965·Journal of Molecular Biology·A O STRETTON, S BRENNER
Jun 1, 1965·Journal of Molecular Biology·G W NOTANIN D ZINDER

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Citations

Aug 5, 1975·Molecular & General Genetics : MGG·J F Atkins, R F Gesteland
May 15, 1980·Journal of Molecular Biology·D IserentantW Fiers
Oct 20, 1977·Microbiology and Immunology·A HirashimaI Watanabe
Apr 1, 1997·Proceedings of the National Academy of Sciences of the United States of America·A HigashitaniK Horiuchi
Nov 25, 1981·Journal of Molecular Biology·M P Calos, J H Miller
Dec 1, 1968·Virology·J R Scott
Sep 1, 1970·Virology·K Horiuchi, S Matsuhashi
Sep 11, 1975·Journal of Virology·D H Walker, J T Walker
Apr 1, 1973·Journal of Virology·A Palmenberg, P Kaesberg

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