Azurocidin is a novel antigen for anti-neutrophil cytoplasmic autoantibodies (ANCA) in systemic vasculitis.

Clinical and Experimental Immunology
M H Zhao, C M Lockwood

Abstract

We isolated a 27-kD protein using cation exchange chromatography from an acid extract of neutrophil granules. N-terminal amino acid sequence analysis of the first 10 residues showed that this protein is azurocidin, a member of the family of neutral serine proteinase found in the neutrophil, which shares amino acid sequence homology with the three other neutral serine proteinases, elastase, proteinase 3 (PR3) and cathepsin G, but unlike them is without proteolytic activity. To test whether, in addition to these proteases, azurocidin might be a target for the humoral autoimmune responses associated with human vasculitis, 185 indirect immunofluorescence (IIF)-positive ANCA sera, made up of four groups of sera with specificities for PR3 (n=37), myeloperoxidase (MPO; n=50), bactericidal/permeability-increasing protein (BPI; n=41) and sera that recognized none of them (triple negative, n=57), and 46 normal sera were screened for IgG anti-azurocidin antibodies using an ELISA incorporating purified azurocidin. Twenty of the 185 IIF-positive sera and 2/46 normal sera displayed reactivity with azurocidin. Positive sera could blot the 27-kD band by Western blot analysis. Further study of the 20 positive sera revealed that: (i) 10 also had...Continue Reading

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Citations

Sep 29, 1998·Arthritis and Rheumatism·G S Hoffman, U Specks
Oct 1, 1998·Autoimmunity·A M FordeJ Jackson
Mar 21, 2002·Expert Review of Molecular Diagnostics·J Savige
Jul 16, 2008·Clinical Reviews in Allergy & Immunology·Birgit Terjung, Ulrich Spengler
Jan 8, 2008·Clinical Reviews in Allergy & Immunology·C BeauvillainA Chevailler
May 19, 2020·Journal of Leukocyte Biology·Martina SundqvistKelly L Brown
Aug 8, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·B TerjungU Spengler
Jan 5, 2021·Frontiers in Immunology·Despina MichailidouChristian Lood
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Sep 21, 2002·Lancet·Robin KahnDiana Karpman
Jan 26, 1999·Clinics in Chest Medicine·E J Sullivan, G S Hoffman

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