B- and T-cell autoantigens in pristane-induced arthritis

Immunology
R N BarkerS J Thompson

Abstract

Pristane-induced arthritis (PIA) is a murine disease resembling rheumatoid arthritis (RA) which is characterized by autoimmune responses to joint tissues. To identify the range of potential antigens targeted in PIA, proteins from arthritic or normal joint extracts were fractionated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and systematically screened for the ability to react with either serum IgG, or cultured splenic T cells, obtained from healthy or arthritic mice. Extracts from both normal and arthritic animals contained multiple proteins that were capable of reacting with murine serum IgG in immunoblotting experiments. In healthy controls, more bands were identified in extracts prepared from 30-week-old mice than from 8-week-old animals, but the widest range of proteins bound were derived from arthritic joints. Furthermore, the sera from PIA-positive mice reacted with more bands from each of the extracts than did normal sera. Fractionated extracts prepared from healthy joints failed to stimulate the in vitro proliferation of splenic T cells from either normal or arthritic animals. When arthritic joint components were screened, T cells from healthy mice responded weakly to some fractions, but mu...Continue Reading

References

Apr 1, 1991·European Journal of Immunology·G RookT Rademacher
Nov 1, 1990·European Journal of Immunology·S J ThompsonC J Elson
Sep 27, 1986·Journal of Immunological Methods·P Laing
Apr 1, 1987·Scandinavian Journal of Immunology·A E BedwellC E Hinton
Nov 1, 1981·Arthritis and Rheumatism·D E TrenthamJ R David
Jan 1, 1996·European Journal of Immunology·F E PerryC J Elson
Aug 1, 1995·Veterinary Immunology and Immunopathology·R N Barker, C J Elson
Feb 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·O S BirkD M Altmann
Jan 1, 1980·Scandinavian Journal of Immunology·T GlantT Szücs

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Citations

Aug 8, 2002·Clinical and Experimental Immunology·R N BarkerC J Elson
Jun 1, 1997·Immunology·L SvelanderS Kleinau
Mar 23, 2004·Annual Review of Immunology·Georg WickQingbo Xu
Jul 16, 2016·Immunologic Research·Jiram Torres RuizYehuda Shoenfeld
May 10, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Marisa BenagianoGianfranco Del Prete
Jan 17, 2019·Journal of Immunology Research·Jussara Gonçalves FernandesMarcelo De Franco
Jan 9, 1999·Clinical Microbiology Reviews·U Zügel, S H Kaufmann

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