Dec 3, 2008

B cell helper assays

Methods in Molecular Biology
Sergio AbrignaniPaolo Dellabona

Abstract

Activation, proliferation and differentiation of naïve B lymphocytes into memory B cells and plasma cells requires engagement of the B cell receptor (BCR) coupled to T-cell help (1, 2). T cells deliver help in cognate fashion when they are activated upon recognition of specific MHC-peptide complexes presented by B cells. T cells can also deliver help in a non-cognate or bystander fashion, when they do not find specific MHC-peptide complexes on B cells and are activated by alternative mechanisms. T-cell dependent activation of B cells can be studied in vitro by experimental models called "B cell helper assays" that are based on the co-culture of B cells with activated T cells. These assays allow to decipher the molecular bases for productive T-dependent B cell responses. We show here examples of B cell helper assays in vitro, which can be reproduced with any subset of T lymphocytes that displays the appropriate helper signals.

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Mentioned in this Paper

T-Lymphocyte
Invariant Natural Killer T-Cells
Receptors, Antigen, B-Cell
Coculture Techniques
Complex (molecular entity)
Cell Differentiation Process
B-Lymphocytes
Cell Separation
Plasma Cells
Clone Cells

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