B-cell populations are expanded in breast cancer patients compared with healthy controls

Breast Cancer : the Journal of the Japanese Breast Cancer Society
Banri TsudaYutaka Tokuda

Abstract

Historically, humoral immunity was considered unimportant in anti-tumor immunity, and the differentiation and anti-tumor activity of B cells in breast cancer are poorly understood. However, it was recently discovered that B cells participate in tumor immunity through both antibody production and immunosuppressive mechanisms. We analyzed the expression of B-cell differentiation markers in detail using fluorescence-activated cell sorting to investigate the relationship between B-cell subsets and breast cancer etiology. Blood samples were taken from breast cancer patients and healthy donors, and peripheral blood mononuclear cells were collected. B cells at various stages of differentiation were identified by the expression of combinations of the cell surface markers CD5, CD19, CD21, CD24, CD27, CD38, CD45, and IgD. Statistical analysis of the proportions of each B-cell subtype in the different patient groups was then performed. Twenty-seven breast cancer patients and 12 controls were considered. The proportion of total B cells was significantly higher in cancer patients than in controls (11.51 ± 2.059 vs 8.905 ± 0.379%, respectively; p = 0.001). Breast cancer patients were then classified as High-B or Low-B for further analysis. A...Continue Reading

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Methods Mentioned

BETA
fluorescence-activated cell sorting
flow cytometry
FACS

Related Concepts

Plasma Cell Antigens PC-1
Mammary Neoplasms, Human
Cell Differentiation Process
Cell Separation
Flow Cytometry
Viral Markers
Be2 Cells
B-Lymphocytes
Biological Markers
Malignant Neoplasm of Breast

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