B cell repertoire in adult antigen-free and conventional neonatal BALB/c mice. I. Preferential utilization of the CH-proximal VH gene family PC7183.

European Journal of Immunology
N A Bos, C G Meeuwsen

Abstract

Early in ontogeny B cells preferentially use VH gene families which are most adjacent to the genes coding for the constant part of the immunoglobulin molecule. In conventional adult mice, however, a random usage of VH gene families has been found. We investigated the role of exogenous antigenic stimulation on this normalization of VH gene usage by B cells. Therefore, we made use of adult germ-free BALB/c mice fed a chemically defined ultrafiltered antigen-free diet (GF-CD) and neonatal conventional BALB/c mice. Both the adult GF-CD and the newborn conventional mice represent situations with minimal exogenous antigenic stimulation. The results obtained with RNA dot blot hybridization with probes specific for the different VH gene families showed in hybridomas from adult GF-CD BALB/c mice a preferential usage of the CH-proximal VH gene family PC7183. In hybridomas from 5-day-old conventional BALB/c mice a less frequent usage of the J558 VH gene family was found and an increased usage of the PC7183 VH gene family than what would be expected from random usage. Evidence is presented that the RNA giving a positive signal with the PC7183 probe represents functional messages for IgM production.

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Citations

Sep 1, 1990·Journal of Clinical Immunology·C A BonaG Kelsoe
May 1, 1995·Research in Immunology·S Avrameas, T Ternynck
Jun 19, 2001·Journal of Immunological Methods·A M SeijenN A Bos
Jun 29, 2002·Current Opinion in Immunology·Rolf M Zinkernagel
Aug 23, 2002·Immunological Reviews·Rolf M Zinkernagel
Dec 21, 2006·Nature Immunology·Rolf Zinkernagel
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·H W Schroeder, J Y Wang
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Jan 1, 1994·Viral Immunology·I M FernándezH Snippe
Apr 7, 2012·Cellular and Molecular Life Sciences : CMLS·Rolf M Zinkernagel
Jun 21, 2015·Immunology Letters·Fabio R Santori
Aug 26, 2004·Blood·Jeyarani RegunathanSubramaniam Malarkannan
Apr 10, 2010·International Journal of Immunopathology and Pharmacology·M A AvanziniM Marconi

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