B cell subset distribution in human bone marrow is stable and similar in left and right femur: An instructive case

PloS One
Annika WiedemannThomas Dörner

Abstract

The bone marrow (BM) is, in addition to being the site of B cell development, a tissue that harbors long-lived plasma cells (PC), the cells that protect the body against foreign antigens by continuous production of antibodies. Nothing is known about the long-term stability and functionality of both B cells and PC in the BM at the individual donor level since repeated sampling possibilities outside of oncology are scarce. Here, we had the opportunity to obtain BM samples from a patient undergoing bilateral total hip arthroplasty half a year apart. We observed that the frequencies of the analyzed B cell and PC subsets were similar despite a time of six months in between and sampling on left and right side of the body. Additionally, B cell receptor stimulation led to comparable results. Our data suggest that composition and functionality of B cells are stable in the BM of adults at the individual donor level.

References

Jun 8, 1999·Annual Review of Immunology·T Kurosaki
Aug 7, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Giuliana CasseseRudolf A Manz
Jan 11, 2011·Nature Immunology·Van Trung ChuClaudia Berek
Apr 4, 2013·Blood·Dora PintoDavid Jarrossay
Feb 26, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Claudia GieseckeThomas Dörner
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Jan 4, 2018·Blood Advances·Siggeir F BrynjolfssonMary-Jo Wick

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Methods Mentioned

BETA
total hip arthroplasty
density gradient centrifugation
FACS
flow-cytometry

Software Mentioned

GraphPad Prism
FlowJo

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