B cells contribute to heterogeneity of IL-17 producing cells in rheumatoid arthritis and healthy controls

PloS One
Paul Martin SchlegelClaudia A Müller

Abstract

Secretion of the proinflammatory cytokine Interleukin-17A (IL-17A) is the hallmark of a unique lineage of CD4 T cells designated Th17 cells, which may play a crucial role in the pathogenesis of rheumatoid arthritis (RA) and many autoimmune diseases. Recently, IL-17-producing cells other than T cells have been described, including diverse innate immune cells. Here, we show that the cellular sources of IL-17A in RA include a significant number of non-T cells. Multicolour fluorescence analysis of IL-17-expressing peripheral blood mononuclear cells (PBMC) revealed larger proportions of IL-17(+)CD3(-) non-T cells in RA patients than in healthy controls (constitutive, 13.6% vs. 8.4%, and after stimulation with PMA/ionomycin 17.4% vs. 7.9% p < 0.001 in both cases). The source of IL-17 included CD3(-)CD56(+) NK cells, CD3(-)CD14(+) myeloid cells as well as the expected CD3(+)CD4(+) Th17 cells and surprisingly a substantial number of CD3(-)CD19(+) B cells. The presence of IL-17A-expressing B cells was confirmed by specific PCR of peripheral MACS-sorted CD19(+) B cells, as well as by the analysis of different EBV-transformed B cell lines. Here we report for the first time that in addition to Th17 cells and different innate immune cells B...Continue Reading

References

Feb 29, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·M ZiolkowskaW Maslinski
Jul 7, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·E LubbertsW B van den Berg
Oct 10, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·S TakemuraC M Weyand
May 16, 2002·Current Rheumatology Reports·Wim B Van den Berg
Jan 15, 2003·Annals of the Rheumatic Diseases·X MarietteR Kimberly
Mar 9, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Martin E DuddyAmit Bar-Or
Jan 12, 2005·Arthritis Research & Therapy·Erik LubbertsWim B van den Berg
Mar 8, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Shannon K O'NeillAlison Finnegan
Nov 25, 2005·Scandinavian Journal of Immunology·P Szodoray, R Jonsson
Jan 5, 2007·Annual Review of Immunology·Casey T WeaverLaurie E Harrington
Mar 7, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Masayuki UmemuraGoro Matsuzaki
May 2, 2007·The Journal of Experimental Medicine·Marie-Laure MichelMaria C Leite-de-Moraes
Dec 8, 2007·Annals of the Rheumatic Diseases·H YamadaY Yoshikai
Jan 8, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Federica CapolunghiRita Carsetti
Apr 29, 2008·Current Opinion in Immunology·Christina L RoarkRebecca L O'Brien
Aug 8, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jill F WrightBeatriz M Carreno
Aug 20, 2008·Arthritis Research & Therapy·Shiva ShahraraRichard M Pope
Aug 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Irina GavanescuDiane Mathis
Sep 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Queenie Lai Kwan LamLiwei Lu
Dec 31, 2008·The Journal of Experimental Medicine·Hiroaki TakatoriJohn J O'Shea
Feb 10, 2009·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Xiaoli LiuLing Chen
Feb 10, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Takaaki KondoMasafumi Takiguchi
Mar 14, 2009·European Journal of Immunology·Rebecca L O'BrienWilli K Born
Aug 19, 2009·Basic & Clinical Pharmacology & Toxicology·Riku Korhonen, Eeva Moilanen
Jan 22, 2010·Current Opinion in Rheumatology·Bethany MarstonJennifer H Anolik
Mar 5, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Axel J HueberIain B McInnes

❮ Previous
Next ❯

Citations

May 31, 2014·BioMed Research International·Serena BugattiAntonio Manzo
Jul 31, 2014·The Journal of Experimental Medicine·Natalia F SmirnovaMuriel Laffargue
Mar 17, 2015·International Reviews of Immunology·Wen-Xing LiuWei-Min Deng
Oct 21, 2015·Nature Reviews. Rheumatology·Louisa E JefferyMartin Hewison
Aug 6, 2015·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Priyanka GaurAmita Aggarwal
Aug 13, 2015·AMB Express·Seiji ShibasakiTsuyoshi Iwasaki
Jan 26, 2017·Gene·Kong Chen, Jay K Kolls
Aug 17, 2018·Nutrition & Dietetics : the Journal of the Dietitians Association of Australia·Nasim KhatibiAmin Salehi-Abargouei
Nov 16, 2019·The Journal of Experimental Medicine·Pascale ZwickyBurkhard Becher
Feb 23, 2017·Microbiology and Immunology·Edwin HoePaul V Licciardi
Aug 8, 2018·Allergologia et immunopathologia·Aline Aparecida de Lima LiraJefferson Russo Victor
Jan 29, 2020·Biochimica Et Biophysica Acta. Molecular Basis of Disease·María Carolina Amezcua VeselyEva Virginia Acosta Rodríguez
Jun 26, 2021·Frontiers in Immunology·Rick WilbrinkGwenny M Verstappen
Jul 11, 2021·The Journal of Immunology : Official Journal of the American Association of Immunologists·Guillermo Carvajal AlegriaPierre Pochard

❮ Previous
Next ❯

Methods Mentioned

BETA
blood drawing
FCS
density gradient centrifugation
FACS
PCR

Software Mentioned

GraphPad Prism
Flow Jo
GraphPad
TreeStar
FACSDiva

Related Concepts

Related Feeds

Cancer Biology: Molecular Imaging

Molecular imaging enables noninvasive imaging of key molecules that are crucial to tumor biology. Discover the latest research in molecular imaging in cancer biology in this feed.

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.

Related Papers

The Journal of Immunology : Official Journal of the American Association of Immunologists
Axel J HueberIain B McInnes
Current Opinion in Immunology
Christina L RoarkRebecca L O'Brien
Journal of Neuroinflammation
Laila Yousef Al-Ayadhi, Gehan Ahmed Mostafa
© 2021 Meta ULC. All rights reserved